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T1 Effects of Low Phytanic Acid-Concentrated DHA on Activated Microglial Cells: Comparison with a Standard Phytanic Acid- Concentrated DHA
A1 Ruiz-Roso, María Belén
A1 Olivares Álvaro, Elena
A1 Quintela, José Carlos
A1 Ballesteros Rodrigálvarez, Sandra María
A1 Espinosa Parrilla, Juan F.
A1 Ruiz-Roso Calvo De La Mora, Baltasar
A1 Lahera Julia, Vicente
A1 Heras Jiménez, Natalia De Las
A1 Martín Fernández, Beatriz
AB Docosahexaenoic acid (DHA, 22:6 n-3) is an essential omega-3 (ω-3) long chain polyunsaturated fatty acid of neuronal membranes involved in normal growth, development, and function. DHA has been proposed to reduce deleterious effects in neurodegenerative processes. Even though, some inconsistencies in findings from clinical and pre-clinical studies with DHA could be attributed to the presence of phytanic acid (PhA) in standard DHA treatments. Thus, the aim of our study was to analyze and compare the effects of a low PhA-concentrated DHA with a standard PhA-concentrated DHA under different neurotoxic conditions in BV-2 activated microglial cells. To this end, mouse microglial BV-2 cells were stimulated with either lipopolysaccharide (LPS) or hydrogen peroxide (H2O2) and co-incubated with DHA 50 ppm of PhA (DHA (PhA:50)) or DHA 500 ppm of PhA (DHA (PhA:500)). Cell viability, superoxide anion (O2-) production, Interleukin 6 (L-6), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), glutathione peroxidase (GtPx), glutathione reductase (GtRd), Caspase-3, and the brain-derived neurotrophic factor (BDNF) protein expression were explored. Low PhA-concentrated DHA protected against LPS or H2O2-induced cell viability reduction in BV-2 activated cells and O2- production reduction compared to DHA (PhA:500). Low PhA-concentrated DHA also decreased COX-2, IL-6, iNOS, GtPx, GtRd, and SOD-1 protein expression when compared to DHA (PhA:500). Furthermore, low PhA-concentrated DHA increased BDNF protein expression in comparison to DHA (PhA:500). The study provides data supporting the beneficial effect of low PhA-concentrated DHA in neurotoxic injury when compared to a standard PhA-concentrated DHA in activated microglia.
PB Springer Nature
SN 1559-1174
YR 2018
FD 2018-05-30
LK https://hdl.handle.net/20.500.14352/133597
UL https://hdl.handle.net/20.500.14352/133597
LA eng
NO Ruiz-Roso MB, Olivares-Álvaro E, Quintela JC, Ballesteros S, Espinosa-Parrilla JF, Ruiz-Roso B, Lahera V, de Las Heras N, Martín-Fernández B. Effects of Low Phytanic Acid-Concentrated DHA on Activated Microglial Cells: Comparison with a Standard Phytanic Acid-Concentrated DHA. Neuromolecular Med. 2018 Sep;20(3):328-342. doi: 10.1007/s12017-018-8496-8. Epub 2018 May 30. PMID: 29846873.
NO Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad, Plan Estatal de Investigación Científica y Técnica y de Innovación 2013–2016
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 18 mar 2026