RT Journal Article
T1 Sperm metabolomics through nuclear magnetic resonance spectroscopy
A1 Lombó, Marta
A1 Ruiz Díaz, Sara
A1 Gutiérrez Adán, Alfonso
A1 Sánchez Calabuig, María Jesús
A2 MDPI, 
AB This report reviews current knowledge of sperm metabolomics analysis using proton nuclear magnetic resonance spectroscopy (1 H-NMR) with particular emphasis on human and farm animals. First, we present the benefits of NMR over other techniques to identify sperm metabolites and then describe the specific methodology required for NMR sperm analysis, stressing the importance of analyzing metabolites extracted from both the hydrophilic and lipophilic phases. This is followed by a description of advances produced to date in the use of NMR to diagnose infertility in humans and to identify metabolic differences among the sperm of mammalian herbivore, carnivore, and omnivore species. This last application of NMR mainly seeks to explore the possible use of lipids to fuel sperm physiology, contrary to previous theories that glycolysis and oxidative phosphorylation (OXPHOS) are the only sources of sperm energy. This review describes the use of NMR to identify sperm and seminal plasma metabolites as possible indicators of semen quality, and to examine the metabolites needed to maintain sperm motility, induce their capacitation, and consequently, to predict animal fertility.
PB MDPI
SN 2076-2615
YR 2021
FD 2021-06-03
LK https://hdl.handle.net/20.500.14352/107259
UL https://hdl.handle.net/20.500.14352/107259
LA eng
NO Lombó, M.; Ruiz-Díaz, S.; Gutiérrez-Adán, A.; Sánchez-Calabuig, M.-J. Sperm Metabolomics through Nuclear Magnetic Resonance Spectroscopy. Animals 2021, 11, 1669. https:// doi.org/10.3390/ani11061669
NO AGL2015-73249-JIN(AEI/FEDER/UE)
DS Docta Complutense
RD 21 abr 2025