RT Journal Article
T1 Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing
A1 Tejerina Miranda, Sandra
A1 Pedrero Muñoz, María
A1 Blázquez García, Marina
A1 Serafín González-Carrato, Verónica
A1 Montero Calle, Ana
A1 Garranzo Asensio, María
A1 Reviejo García, Ángel Julio
A1 Pingarrón Carrazón, José Manuel
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
AB This work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytical biotool combines the benefits of using magnetic microparticles for the implementation of sandwich immunoassays and amperometric transduction on disposable carbon electrodes. A limit of detection (LOD) of 34.1 pg mL−1 for HE standards and a selectivity suitable for its foray into the clinical oncology area, are demonstrated. The determination of HE in clinical samples such as lysates and secretomes of colorectal cancer (CRC) cells, plasma, and tissue samples from patients with CRC in different stages, has been faced with satisfactory results showing the ability for discriminating the metastatic capabilities of cells and for identifying and staging CRC patients. The developed bioplatform allows precise quantitative determinations, requiring minimal pre-treatments and sample amounts in only 75 min. In addition, due to the instrumentation and the type of substrates used in the detection step, the biotool is compatible with implementation in multiplexed and/or point-of-need devices, features in which this bioplatform is advantageous with respect to the enzyme linked immunosorbent assay (ELISA) or immunoblotting technologies.
PB Elsevier
SN 1567-5394
YR 2024
FD 2024-02
LK https://hdl.handle.net/20.500.14352/105630
UL https://hdl.handle.net/20.500.14352/105630
LA eng
NO andra Tejerina-Miranda, María Pedrero, Marina Blázquez-García, Verónica Serafín, Ana Montero-Calle, Maria Garranzo-Asensio, A. Julio Reviejo, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano, Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing, Bioelectrochemistry, Volume 155, 2024, 108571, ISSN 1567-5394, https://doi.org/10.1016/j.bioelechem.2023.108571.
DS Docta Complutense
RD 20 jul 2024