RT Journal Article
T1 Nisin S, a Novel Nisin Variant Produced by Ligilactobacillus salivarius P1CEA3
A1 Sevillano, Ester
A1 Peña Vidal, Nuria
A1 Lafuente, Irene
A1 Cintas Izarra, Luis Miguel
A1 Muñoz Atienza, Estefanía
A1 Hernández Cruza, Pablo Elpidio
A1 Borrero Del Pino, Juan
AB Recently, the food industry and the animal farming field have been working on different strategies to reduce the use of antibiotics in animal production. The use of probiotic producers of antimicrobial peptides (bacteriocins) is considered to be a potential solution to control bacterial infections and to reduce the use of antibiotics in animal production. In this study, Ligilactobacillus salivarius P1CEA3, isolated from the gastrointestinal tract (GIT) of pigs, was selected for its antagonistic activity against Gram-positive pathogens of relevance in swine production. Whole genome sequencing (WGS) of L. salivarius P1ACE3 revealed the existence of two gene clusters involved in bacteriocin production, one with genes encoding the class II bacteriocins salivaricin B (SalB) and Abp118, and a second cluster encoding a putative nisin variant. Colony MALDI-TOF MS determinations and a targeted proteomics combined with massive peptide analysis (LC-MS/MS) of the antimicrobial peptides encoded by L. salivarius P1CEA3 confirmed the production of a 3347 Da novel nisin variant, termed nisin S, but not the production of the bacteriocins SalB and Abp118, in the supernatants of the producer strain. This is the first report of a nisin variant encoded and produced by L. salivarius, a bacterial species specially recognized for its safety and probiotic potential.
PB MDPI
SN 1422-0067
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/109088
UL https://hdl.handle.net/20.500.14352/109088
LA eng
NO Sevillano, E., Peña, N., Lafuente, I., Cintas, L. M., Muñoz-Atienza, E., Hernández, P. E., & Borrero, J. (2023). Nisin s, a novel nisin variant produced by ligilactobacillus salivarius p1cea3. International Journal of Molecular Sciences, 24(7), 6813. https://doi.org/10.3390/ijms24076813
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 6 abr 2025