RT Journal Article
T1 Assessing Chronodisruption Distress in Goldfish: The Importance of Multimodal Approaches
A1 Saiz Aparicio, Nuria
A1 Herrera-Castillo, Lisbeth
A1 Pedro Ormeño, Nuria De
A1 Delgado Saavedra, María Jesús
A1 Arvidsson, Sven David
A1 Marugal-López, Miguel Ángel
A1 Isorna Alonso, Esther
A2 MDPI, 
AB Chronodisruption caused by factors such as light at night and mistimed meals has been linked to numerous physiological alterations in vertebrates and may be an anxiogenic factor affecting welfare. This study aims to investigate whether chronodisruption causes measurable changes in the anxiety responses of goldfish under two conditions: randomly scheduled feeding (RF) and continuous light (LL). Anxiety-like behavior was assessed in the open field with object approach and black/white preference tests, which had been validated using diazepam. An increased thigmotaxis response and decreased object exploration under both chronodisruption protocols indicated anxiety states. Furthermore, locomotor activity was increased in LL fish. The black/white preference test discriminated anxiolysis induced by diazepam but was unable to detect anxiety caused by chronodisruption. Plasma cortisol increased in both RF and LL fish throughout the experiment, confirming that both conditions caused stress. The LL fish also showed an apparently desensitized hypothalamus–pituitary–interrenal HPI axis, with a decrease in pomc and crf expression. Individual analysis found no correlation between anxiety-like behavior and stress axis activation nor between scototaxis and thigmotaxis responses. However, individual differences in sensitivity to each test were detected. Altogether, these results highlight circadian disruption as a stressor for fish and endorse a multiple variable approach for reliably assessing animal discomfort.
PB MDPI
SN 2076-2615
YR 2023
FD 2023-08-01
LK https://hdl.handle.net/20.500.14352/103581
UL https://hdl.handle.net/20.500.14352/103581
LA eng
NO Saiz, N., Herrera-Castillo, L., de Pedro, N., Delgado, M. J., Arvidsson, S. D., Marugal-López, M. Á., & Isorna, E. (2023). Assessing Chronodisruption Distress in Goldfish: The Importance of Multimodal Approaches. Animals : an open access journal from MDPI, 13(15), 2481. https://doi.org/10.3390/ani13152481
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Complutense de Madrid (España)
DS Docta Complutense
RD 26 ago 2024