RT Journal Article
T1 Intermolecular Interaction Between Cry2Aa and Cyt1Aa and Its Effect on Larvicidal Activity Against Culex quinquefasciatus
A1 Bideshi, Dennis
A1 Waldrop, Greer
A1 Fernandez-Luna, Maria Teresa
A1 Díaz Mendoza, María Mercedes
A1 Wirth, Margaret
A1 Johnson, Jeffrey
A1 Park, Hyun-Woo
A1 Federici, Brian
AB The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex quinquefasciatus. It is also known that Cyt1Aa, which is highly lipophilic, synergizes Cry11Aa by functioning as a surrogate membrane-bound receptor for the latter protein. Little is known, however, about whether Cyt1Aa can interact similarly with other Cry proteins not primarily mosquitocidal; for example, Cry2Aa, which is active against lepidopteran larvae, but essentially inactive or has very low toxicity to mosquito larvae. Here we demonstrate by ligand binding and enzyme-linked immunosorbent assays that Cyt1Aa and Cry2Aa form intermolecular complexes in vitro, and in addition show that Cyt1Aa facilitates binding of Cry2Aa throughout the midgut of C. quinquefasciatus larvae. As Cry2Aa and Cry11Aa share structural similarity in domain II, the interaction between Cyt1Aa and Cry2Aa could be a result of a similar mechanism previously proposed for Cry11Aa and Cyt1Aa. Finally, despite the observed interaction between Cry2Aa and Cyt1Aa, only a 2-fold enhancement in toxicity resulted against C. quinquefasciatus. Regardless, our results suggest that Cry2Aa could be a useful component of mosquitocidal endotoxin complements being developed for recombinant strains of B. thuringiensis subsp. israelensis and B. sphaericus aimed at improving the efficacy of commercial products and avoiding resistance.
PB Korean Society for Microbiology and Biotechnology
SN 1017-7825
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/95975
UL https://hdl.handle.net/20.500.14352/95975
LA eng
NO Bideshi, Dennis K. «Intermolecular Interaction Between Cry2Aa and Cyt1Aa and Its Effect on Larvicidal Activity Against Culex quinquefasciatus». Journal of Microbiology and Biotechnology, vol. 23, n.o 8, agosto de 2013, pp. 1107-15. https://doi.org/10.4014/jmb.1301.01062.
NO National Institute of Allergy and Infectious Diseases
DS Docta Complutense
RD 20 abr 2025