%0 Journal Article
%A Barrio Asensio, María Del Carmen
%A Río Sevilla, Aurora Del
%A Murillo González, Jorge Alfonso
%A Maldonado Bautista, Estela
%A López Gordillo, Yamila
%A Paradas Lara, Irene
%A Hernandes, Luzmarina
%A Catón Vázquez, Javier
%A Martínez Álvarez, Concepción
%T Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the T gf-β3 Null Mutant
%D 2014
%@ 1422-6405
%U https://hdl.handle.net/20.500.14352/35215
%X The cleft palate presented by transforming growth factor-β3 (Tgf-β3 ) null mutant mice is caused by altered palatal shelf adhesion, cell proliferation, epithelial-to-mesenchymal transformation and cell death. The  expression of epidermal growth factor (EGF), transforming growth factor-β1 ( Tgf-β1 ) and muscle segment  homeobox-1 (Msx-1) is modified in the palates of these knockout mice, and the cell proliferation defect is caused  by the change in EGF expression. In this study, we aimed to determine whether this change in EGF expression has any effect on the other mechanisms altered in Tgf-β 3 knockout mouse palates. We tested the effect of inhibiting EGF activity in vitro in the knockout palates via the addition of Tyrphostin AG 1478. We also investigated  possible interactions between EGF, Tgf-β 1 and Msx-1 in Tgf-β 3 null mouse palate cultures. The results show that  the inhibition of EGF activity in Tgf-β 3 null mouse palate cultures improves palatal shelf adhesion and fusion,  with a particular effect on cell death, and restores the normal distribution pattern of Msx-1 in the palatal  esenchyme. Inhibition of TGF-β 1 does not affect either EGF or Msx-1 expression.
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