RT Journal Article
T1 Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the T gf-β3 Null Mutant
A1 Barrio Asensio, María Del Carmen
A1 Río Sevilla, Aurora Del
A1 Murillo González, Jorge Alfonso
A1 Maldonado Bautista, Estela
A1 López Gordillo, Yamila
A1 Paradas Lara, Irene
A1 Hernandes, Luzmarina
A1 Catón Vázquez, Francisco Javier
A1 Martínez Álvarez, María Concepción
AB The cleft palate presented by transforming growth factor-β3 (Tgf-β3 ) null mutant mice is caused by altered palatal shelf adhesion, cell proliferation, epithelial-to-mesenchymal transformation and cell death. The  expression of epidermal growth factor (EGF), transforming growth factor-β1 ( Tgf-β1 ) and muscle segment  homeobox-1 (Msx-1) is modified in the palates of these knockout mice, and the cell proliferation defect is caused  by the change in EGF expression. In this study, we aimed to determine whether this change in EGF expression has any effect on the other mechanisms altered in Tgf-β 3 knockout mouse palates. We tested the effect of inhibiting EGF activity in vitro in the knockout palates via the addition of Tyrphostin AG 1478. We also investigated  possible interactions between EGF, Tgf-β 1 and Msx-1 in Tgf-β 3 null mouse palate cultures. The results show that  the inhibition of EGF activity in Tgf-β 3 null mouse palate cultures improves palatal shelf adhesion and fusion,  with a particular effect on cell death, and restores the normal distribution pattern of Msx-1 in the palatal  esenchyme. Inhibition of TGF-β 1 does not affect either EGF or Msx-1 expression.
PB Karger
SN 1422-6405
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/35215
UL https://hdl.handle.net/20.500.14352/35215
LA eng
NO Barrio Asensio, M. C., Rio Sevilla, A., Murillo González, J. A. et al. «Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the Tgf-β3 Null Mutant». Cells Tissues Organs, vol. 199, n.o 2-3, mayo de 2014, pp. 201-11. Silverchair, https://doi.org/10.1159/000362227.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Universidad Complutense
NO Comunidad Autónoma de Madrid
NO Banco Santander
DS Docta Complutense
RD 29 sept 2024