RT Journal Article T1 Aging dependent effect of nuclear tau A1 Montoya Miñano, Juan José A1 Gil, Laura A1 Concetta, Federico A1 Pinedo, Fernando A1 Bruno, Francesca A1 Rebolledo, Ana B. A1 Olazabal, Isabel M. A1 Ferrer, Isidre A1 Saccone, Salvatore AB Tau protein is characterized by a complex pattern of phosphorylation and is localized in the cytoplasm and nucleus in both neuronal and non-neuronal cells. Human AT100 nuclear tau, endowed by phosphorylation in Thr212/Ser214, was recently shown to decline in cornus ammonis 1 (CA1) and dentate gyrus (DG) in Alzheimer's disease (AD), but a defined function for this nuclear tau remains unclear. Here we show that AT100 progressively increases in the nuclei of neuronal and non-neuronal cells during aging, and decreases in the more severe AD stages, as recently shown, and in cancer cells (colorectal adenocarcinoma and breast cancer). AT100, in addition to a co-localization with the DAPI-positive heterochromatin, was detected in the nucleolus of pyramidal cells from the CA1 region, shown to be at its highest level in the more senescent cells and in the first stage of AD (ADI), and disappearing in the more severe AD cases (ADIV). Taking into account the nuclear distribution of AT100 during cell aging and its relation to the chromatin changes observed in degenerated neurons, as well as in cancerous cells, which are both cellular pathologies associated with age, we can consider the Thr212/Ser214 phosphorylated nuclear tau as a molecular marker of cell aging. PB Science Direct YR 2017 FD 2017-09-30 LK https://hdl.handle.net/20.500.14352/114893 UL https://hdl.handle.net/20.500.14352/114893 LA eng NO Gil, L., Federico, C., Pinedo, F., Bruno, F., Rebolledo, A. B., Montoya, J. J., Olazabal, I. M., Ferrer, I., & Saccone, S. (2017). Aging dependent effect of nuclear tau. Brain Research, 1677, 129-137. https://doi.org/10.1016/J.BRAINRES.2017.09.030 NO Biobanco of Hospital Universitario Fundación Alcorcón NO Foundation Universidad Alfonso X el Sabio NO University of Catania DS Docta Complutense RD 18 abr 2025