RT Journal Article
T1 Effects of Extender Type, Storage Time, and Temperature on Bull Semen Parameters
A1 Fernandez Novo, Aitor
A1 Santos López, Sergio
A1 Barrajon Masa, Clara
A1 Mozas, Patricia
A1 de Mercado, Eduardo
A1 Caceres, Elisa
A1 Garrafa, Aizic
A1 González Martín, Juan Vicente
A1 Perez Villalobos, Natividad
A1 Oliet, Agustin
A1 Astiz, Susana
A1 Perez Garnelo, Sonia Salome
AB Seminal parameters can be evaluated in situ, or samples can be delivered to a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on semen quality. Acrosome integrity, sperm viability and morphology, CASA-total and progressive motility, pH, and colony-forming units were assessed. Semen quality was preserved for up to 4–6 h post-ejaculation, except for INRA96® at 5 °C. Regardless of extender or temperature, motility decreased from 4 to 6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. pH differed from 4 to 6 h up to 24 h, acidifying when stored at room temperature. Microbiological load was stable over time with AndroMed® and BIOXcell®, and increased at room temperature with INRA96®. Our results suggest that AndroMed® and BIOXcell® can preserve semen quality for up to 6 h, either at 5 °C or room temperature, while INRA96® only at room temperature. These results help to fix adequate protocols for short-term storage and shipment of bovine semen collected under field conditions.
PB MDPI
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/115905
UL https://hdl.handle.net/20.500.14352/115905
LA eng
NO Fernandez-Novo, A., Santos-Lopez, S., Barrajon-Masa, C., Mozas, P., de Mercado, E., Caceres, E., Garrafa, A., Gonzalez-Martin, J. V., Perez-Villalobos, N., Oliet, A., Astiz, S., & Perez-Garnelo, S. S. (2021). Effects of Extender Type, Storage Time, and Temperature on Bull Semen Parameters. Biology, 10(7), 630. https://doi.org/10.3390/biology10070630
NO Author Contributions Conceptualization: A.F.-N., S.S.-L., C.B.-M., J.V.G.-M., N.P.-V., A.O., S.A. and S.S.P.-G.; methodology, A.F.-N., S.S.-L., C.B.-M., P.M., E.d.M., E.C., A.G., S.A. and S.S.P.-G.; software, A.F.-N., C.B.-M., P.M., E.d.M., E.C., A.G., A.O. and S.A.; validation, J.V.G.-M., N.P.-V., A.O., S.A. and S.S.P.-G. ; formal analysis, A.F.-N., S.A. and S.S.P.-G.; investigation, A.F.-N., P.M., E.d.M., E.C., A.G., A.O., S.A. and S.S.P.-G.; resources, A.F.-N., S.S.-L., N.P.-V., A.O., S.A. and S.S.P.-G.; data curation, A.F.-N., S.A. and S.S.P.-G.; writing—original draft preparation, A.F.-N., S.A. and S.S.P.-G.; writing—review and editing, A.F.-N., J.V.G.-M., N.P.-V., S.A. and S.S.P.-G.; visualization, S.A. and S.S.P.-G.; supervision, S.S.-L., J.V.G.-M., N.P.-V., S.A. and S.S.P.-G.; project administration, S.A. and S.S.P.-G.; funding acquisition, S.A. and S.S.P.-G. All authors have read and agreed to the published version of the manuscript.
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria(España)
DS Docta Complutense
RD 7 abr 2025