RT Journal Article
T1 Expression of endothelial nitric oxide synthase in human peritoneal tissue: regulation by escherichia coli lipopolysaccharide
A1 Arriero, María M.
A1 Rodríguez-Feo, Juan A.
A1 Celdrán, Ángel
A1 Sánchez de Miguel, Lourdes
A1 González Fernández, Fernando
A1 Fortes, José
A1 Reyero, Ana
A1 Frieyro, Octavio
A1 Pinta, Juan C de la
A1 Franco, Ángeles
A1 Casado, Santos
A1 López Farre, Antonio José
A1 Pastor Vargas, Carlos
AB Changes in the expression of endothelial nitric oxide synthase (eNOS) in the peritoneum could be involved in the peritoneal dysfunction associated with peritoneal inflammation. Demonstrated recently in bovine endothelial cells was the existence of cytosolic proteins that bind to the 3′-untranslated region (3′-UTR) of eNOS mRNA and could be implicated in eNOS mRNA stabilization. The present work demonstrates that eNOS protein is expressed in human endothelial and mesothelial peritoneal cells. <jats:italic toggle="yes">Escherichia coli</jats:italic> lipopolysaccharide shortened the half-life of eNOS message, reducing eNOS protein expression in peritoneal mesothelial and endothelial cells. Moreover, under basal conditions, human peritoneal samples expressed cytosolic proteins that bind to the 3′-UTR of eNOS mRNA. The cytosolic proteins that directly bind to 3′-UTR were identified as a 60-kD protein. After incubation of human peritoneal samples with lipopolysaccharide, the binding activity of the cytosolic 60-kD protein increased in a time-dependent manner. Studies are now necessary to determine the involvement of this 60-kD protein in the regulation of eNOS expression in peritoneal cells and particularly its involvement in the peritoneal dysfunction associated with inflammatory reactions.
PB Wolters Kluwer
SN 1046-6673
YR 2000
FD 2000-10
LK https://hdl.handle.net/20.500.14352/105612
UL https://hdl.handle.net/20.500.14352/105612
LA eng
NO Arriero MM, Rodríguez-Feo JA, Celdrán Á, Miguel LS, González-Fernández F, Fortes J, Reyero A, Frieyro O, Pinta JC, Franco A, Pastor C, Casado S, López-Farré A. Expression of endothelial nitric oxide synthase in human peritoneal tissue: regulation by Escherichia coli lipopolysaccharide. J Am Soc Nephrol. 2000 Oct;11(10):1848-1856. doi: 10.1681/ASN.V11101848. PMID: 11004215.
DS Docta Complutense
RD 17 abr 2025