RT Journal Article
T1 Electrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode
A1 Serafín González-Carrato, Verónica
A1 Hernández, Paloma
A1 Agüí Chicharro, María Lourdes
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB A composite electrode consisted of gold nanoparticles (AuNPs), multi-walled carbon nanotubes (MWCNTs) and Teflon, to which peroxidase (HRP) and ferrocene (Fc) were incorporated as auxiliary enzyme and redox mediator, respectively, was constructed. The enzymes creatininase, creatinase and sarcosine oxidase were then co-immobilized onto the surface of the resulting HRP/Fc/AuNPs/MWCNTs/Teflon electrode for the preparation of a creatinine biosensor. Amperometry in stirred solutions using a detection potential of 0.0 V vs Ag/AgCl allowed a linear calibration plot to be obtained in the 0.003–1.0 mM creatinine concentration range with a limit of detection of 0.1 M (S/N = 3). The apparent Michaelis-Menten constant for creatininase was KMap= 4.1 ± 0.4 mM. The developed biosensor was validated with good results by determining creatinine in human serum and correlating with the spectrophotometric Jaffe’s method.
PB Elsevier Ltd
SN 0013-4686
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/94510
UL https://hdl.handle.net/20.500.14352/94510
LA eng
NO Serafín V, Hernández P, Agüí L,  Yánez-Sedeño P,  Pingarrón J.M. Electrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode. Electrochim. Acta. 2013 Marzo 13;  97: 175-83
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid 
DS Docta Complutense
RD 29 sept 2024