RT Journal Article
T1 Mesoporous silica nanoparticles containing silver as novel antimycobacterial agents against Mycobacterium tuberculosis
A1 Montalvo Quirós, Sandra
A1 Gómez Graña, Sergio
A1 Vallet Regí, María Dulce Nombre
A1 Prados Rosales, Rafael
A1 González Ortiz, Blanca
A1 Luque García, José Luis
AB Tuberculosis remains today a major public health issue with a total of 9 million new cases and 2 million deaths annually. The lack of an effective vaccine and the increasing emergence of new strains of Mycobacterium tuberculosis (Mtb) highly resistant to antibiotics, anticipate a complicated scenario in the near future. The use of nanoparticles features as an alternative to antibiotics in tackling this problem due to their potential effectiveness in resistant bacterial strains. In this context, silver nanoparticles have demonstrated high bactericidal efficacy,although their use is limited by their relatively high toxicity, which calls for the design of nanocarriers that allow silver based nanoparticles to be safely delivered to the target cells or tissues. In this work mesoporous silica nanoparticles are used as carriers of silver based nanoparticles as antimycobacterial agent against Mtb. Two different synthetic approaches have been used to afford, on the one hand, a 2D hexagonal mesoporous silica nanosystem which contains silver bromide nanoparticles distributed all through the silica network and, on the other hand, a core@shell nanosystem with metallic silver nanoparticles as core and mesoporous silica shell in a radial mesoporous rearrangement. Both materials have demonstrated good antimycobacterial capacity in in vitro test using Mtb, being lower the minimum inhibitory concentration for the nanosystem which contains silver bromide. Therefore, the interaction of this material with the mycobacterial cell has been studied by cryo-electron microscopy, establishing a direct connection between the antimycobactericidal effect observed and the damage induced in the cell envelope.
PB Elsevier
SN 0927-7765
YR 2020
FD 2020-10-04
LK https://hdl.handle.net/20.500.14352/6683
UL https://hdl.handle.net/20.500.14352/6683
LA eng
NO RESEARCH ID N-4501-2014  (Sandra Sánchez Salcedo)ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)RESEARCHER ID G-7562-2016  (Sergio Gómez Graña)ORCID 0000-0002-7736-051X (Sergio Gómez Graña)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)RESEARCHER ID K-4773-2015 (Blanca González Ortiz)ORCID 0000-0002-0493-6071 (Blanca González Ortiz)
NO Unión Europea. Horizonte 2020
NO Ministerio de Ciencia e Innovación (MICINN)
DS Docta Complutense
RD 3 may 2024