RT Book, Section
T1 Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes
A1 Rodrigo Martín Romo, José Augusto
A1 Soto Rueda, Juan Manuel
A1 Alieva Krasheninnikova, Tatiana
AB Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.
PB Society of Photo-Optical Instrumentation Engineers (SPIE)
SN 978-1-5106-2800-7
SN 0277-786X
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/14029
UL https://hdl.handle.net/20.500.14352/14029
LA eng
NO © 2019 Society of Photo-Optical Instrumentation Engineers (SPIE).Optical Methods for Inspection, Characterization, and Imaging of Biomaterials (4º. 2019. Munich)ISSN 0277-786X.The funding of the projects TEC2014-57394-P (Ministerio de Economía y Competitividad) and PR75/18-21567 (Santander-UCM) is acknowledged.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Universidad Complutense de Madrid/Banco de Santander
DS Docta Complutense
RD 20 abr 2025