%0 Journal Article
%A Guerrero Irigoyen, Sara
%A Cadanno Mendía, Dona
%A Agüí Chicharro, María Lourdes
%A Barderas Manchado, Rodrigo
%A Campuzano Ruiz, Susana
%A Yáñez-Sedeño Orive, Paloma
%A Pingarrón Carrazón, José Manuel
%T Click chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serum
%D 2019
%@ 1572-6657
%U https://hdl.handle.net/20.500.14352/92759
%X The first electrochemical immunosensor for the determination of CXCL7 (chemokine (C-X-C motif) ligand 7) autoimmune biomarker is reported in this work. Click chemistry-assisted antibodies immobilization was per formed by reaction of azide functionalized-multi-walled carbon nanotubes (MWCNTs) and ethynyl-IgG onto screen-printed carbon electrodes. The capture antibodies were further immobilized onto IgG-MWCNTs con jugates. After a blocking step with casein, a sandwich immunoassay was implemented involving biotinylated detector antibodies and alkaline phosphatase (AP)-streptavidin conjugate. Differential pulse voltammetry upon addition of 1-naphthylphosphate was used as the analytical readout. A linear calibration plot between 0.5 and 600 pg mL−1 CXCL7 and a LOD value of 0.1 pg mL−1 were obtained. The usefulness of the immunosensor was demonstrated by the successful analysis of serum samples from patients with rheumatoid arthritis.
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