RT Journal Article
T1 Click chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serum
A1 Guerrero Irigoyen, Sara
A1 Cadanno Mendía, Dona
A1 Agüí Chicharro, María Lourdes
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB The first electrochemical immunosensor for the determination of CXCL7 (chemokine (C-X-C motif) ligand 7) autoimmune biomarker is reported in this work. Click chemistry-assisted antibodies immobilization was per formed by reaction of azide functionalized-multi-walled carbon nanotubes (MWCNTs) and ethynyl-IgG onto screen-printed carbon electrodes. The capture antibodies were further immobilized onto IgG-MWCNTs con jugates. After a blocking step with casein, a sandwich immunoassay was implemented involving biotinylated detector antibodies and alkaline phosphatase (AP)-streptavidin conjugate. Differential pulse voltammetry upon addition of 1-naphthylphosphate was used as the analytical readout. A linear calibration plot between 0.5 and 600 pg mL−1 CXCL7 and a LOD value of 0.1 pg mL−1 were obtained. The usefulness of the immunosensor was demonstrated by the successful analysis of serum samples from patients with rheumatoid arthritis.
PB Elsevier
SN 1572-6657
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/92759
UL https://hdl.handle.net/20.500.14352/92759
LA eng
NO Guerrero S, Cadano D, Agüí L, Barderas R, Campuzano S, Yáñez-Sedeño P, et al. Click chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serum. J Electroanal Chem. 2019 Feb 21; 837:246–53.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 7 jun 2025