RT Journal Article
T1 Subtilase Cytotoxin-Coding Genes in Verotoxin-Producing Escherichia coli Strains from Sheep and Goats Differ from Those from Cattle
A1 Carrión Herrero, Francisco Javier
A1 Orden Gutiérrez, José Antonio
A1 Horcajo Iglesias, María Del Pilar
A1 Fuente López, Ricardo De La
A1 Ruiz Santa Quiteria Serrano De La Cruz, José Antonio
A1 Domínguez Bernal, Gustavo Ramón
AB Subtilase cytotoxin (SubAB) from verotoxin (VT)-producing Escherichia coli (VTEC) strains was first described in the 98NK2 strain and has been associated with human disease. However, SubAB has recently been found in two VT-negative E. coli strains (ED 591 and ED 32). SubAB is encoded by two closely linked, cotranscribed genes (subA and subB). In this study, we investigated the presence of subAB genes in 52 VTEC strains isolated from cattle and 209 strains from small ruminants, using PCR. Most (91.9%) VTEC strains from sheep and goats and 25% of the strains from healthy cattle possessed subAB genes. The presence of subAB in a high percentage of the VTEC strains from small ruminants might increase the pathogenicity of these strains for human beings. Some differences in the results of PCRs and in the association with some virulence genes suggested the existence of different variants of subAB. We therefore sequenced the subA gene in 12 strains and showed that the subA gene in most of the subAB-positive VTEC strains from cattle was almost identical (about 99%) to that in the 98NK2 strain, while the subA gene in most of the subAB-positive VTEC strains from small ruminants was almost identical to that in the ED 591 strain. We propose the terms subAB1 to describe the SubAB-coding genes resembling that in the 98NK2 strain and subAB2 to describe those resembling that in the ED 591 strain.
SN 0099-2240
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/93924
UL https://hdl.handle.net/20.500.14352/93924
LA eng
NO Orden, J. A., Horcajo, P., de la Fuente, R., Ruiz-Santa-Quiteria, J. A., Domínguez-Bernal, G., & Carrión, J. (2011). Subtilase cytotoxin-coding genes in verotoxin-producing Escherichia coli strains from sheep and goats differ from those from cattle. Applied and environmental microbiology, 77(23), 8259–8264. https://doi.org/10.1128/AEM.05604-11
NO Banco Santander 
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 14 dic 2025