RT Journal Article
T1 Protective Effect of Thyme and Chestnut Honeys Enriched with Bee Products against Benzo(a)pyrene-Induced DNA Damage
A1 Sánchez Martín, Vanesa
A1 Haza Duaso, Ana Isabel
A1 Iriondo De Hond, Amaia
A1 Del Castillo, María Dolores
A1 Fernández Hospital, Xavier
A1 Fernández Álvarez, Manuela
A1 Hierro Paredes, Eva
A1 Morales Gómez, Paloma
A2 Tchounwou, Paul B.
AB The aim of the present study was to validate the cytotoxicity, genotoxicity, and preventive potential against benzo(a)pyrene (BaP)-induced DNA damage of nine samples of thyme and chestnut honeys enriched with bee products (royal jelly and propolis, 2–10%). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (0–250 mg/mL) to select nontoxic concentrations, and DNA damage (0.1–10 μg/mL) was evaluated by the alkaline single-cell gel electrophoresis or comet assay. Treatment with honey samples or royal jelly and propolis did not affect the viability of HepG2 cells up to 100 and 50 mg/mL, respectively. Treatment with 100 μM BaP significantly increased (p ≤ 0.001) the levels of the DNA strand breaks. None of the tested concentrations (0.1–10 μg/mL) of the honey samples (thyme and chestnut), royal jelly, and propolis caused DNA damage per se. All tested samples at all the concentrations used decreased the genotoxic effect of BaP. In addition, all mixtures of thyme or chestnut honeys with royal jelly or propolis showed a greater protective effect against BaP than the samples alone, being the thyme and chestnut honey samples enriched with 10% royal jelly and 10% propolis the most effective (70.4% and 69.4%, respectively). The observed protective effect may be associated with the phenolic content and antioxidant capacity of the studied samples. In conclusion, the thyme and chestnut honey samples enriched with bee products present potential as natural chemoprotective agents against the chemical carcinogen BaP.
PB MDPI
SN 1660-4601
YR 2022
FD 2022-12-17
LK https://hdl.handle.net/20.500.14352/93434
UL https://hdl.handle.net/20.500.14352/93434
LA eng
NO Sánchez-Martín, V.; Haza, A.I.; Iriondo-DeHond, A.; del Castillo, M.D.; Hospital, X.F.; Fernández, M.; Hierro, E.; Morales, P. Protective Effect of Thyme and Chestnut Honeys Enriched with Bee Products against Benzo(a)pyreneInduced DNA Damage. Int. J. Environ. Res. Public Health 2022, 19, 16969. https://doi.org/10.3390/ ijerph192416969
NO Ministerio de Ciencia e Innovación - Grant RTI2018-097549-B-I00
DS Docta Complutense
RD 2 sept 2024