%0 Journal Article
%A Cea Callejo, Pablo
%A Arca Lafuente, Sonia
%A Gómez-Lucía Duato, María Esperanza
%A Domenech Gómez, Ana María
%A Biarnés, Mar
%A Blanco, Ángela
%A Benítez Rico, Laura
%A Madrid González, Ricardo
%T An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
%D 2024
%@ 0175-7598
%U https://hdl.handle.net/20.500.14352/107361
%X Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming.
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