RT Journal Article
T1 An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
A1 Cea Callejo, Pablo
A1 Arca Lafuente, Sonia
A1 Gómez-Lucía Duato, María Esperanza
A1 Domenech Gómez, Ana María
A1 Biarnés, Mar
A1 Blanco, Ángela
A1 Benítez Rico, Laura
A1 Madrid González, Ricardo
AB Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming.
PB Springer
SN 0175-7598
YR 2024
FD 2024-07-10
LK https://hdl.handle.net/20.500.14352/107361
UL https://hdl.handle.net/20.500.14352/107361
LA eng
NO Cea-Callejo, P., Arca-Lafuente, S., Gomez-Lucia, E. et al. An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus. Appl Microbiol Biotechnol 108, 414 (2024). https://doi.org/10.1007/s00253-024-13243-x
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad Autónoma de Madrid
DS Docta Complutense
RD 6 oct 2024