RT Journal Article
T1 Cux1 and Cux2 Regulate Dendritic Branching, Spine Morphology, and Synapses of the Upper Layer Neurons of the Cortex
A1 Cubelos, Beatriz
A1 Sebastián Serrano, Álvaro
A1 Beccari, Leonardo
A1 Calcagnotto, Maria Elisa
A1 Cisneros, Elsa
A1 Kim, Seonhee
A1 Dopazo, Ana
A1 Alvarez-Dolado, Manuel
A1 Redondo, Juan Miguel
A1 Bovolenta, Paola
A1 Walsh, Christopher A.
A1 Nieto, Marta
AB Dendrite branching and spine formation determines the function of morphologically distinct and specialized neuronal subclasses. However, little is known about the programs instructing specific branching patterns in vertebrate neurons and whether such programs influence dendritic spines and synapses. Using knockout and knockdown studies combined with morphological, molecular, and electrophysiological analysis, we show that the homeobox Cux1 and Cux2 are intrinsic and complementary regulators of dendrite branching, spine development, and synapse formation in layer II-III neurons of the cerebral cortex. Cux genes control the number and maturation of dendritic spines partly through direct regulation of the expression of Xlr3b and Xlr4b, chromatin remodeling genes previously implicated in cognitive defects. Accordingly, abnormal dendrites and synapses in Cux2(-/-) mice correlate with reduced synaptic function and defects in working memory. These demonstrate critical roles of Cux in dendritogenesis and highlight subclass-specific mechanisms of synapse regulation that contribute to the establishment of cognitive circuits.
PB Cell Press
SN 0896-6273
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/96247
UL https://hdl.handle.net/20.500.14352/96247
LA eng
NO Cubelos B, Sebastián-Serrano A, Beccari L, Calcagnotto ME, Cisneros E, Kim S, Dopazo A, Alvarez-Dolado M, Redondo JM, Bovolenta P, Walsh CA, Nieto M. Cux1 and Cux2 regulate dendritic branching, spine morphology, and synapses of the upper layer neurons of the cortex. Neuron. 2010 May 27;66(4):523-35. doi: 10.1016/j.neuron.2010.04.038. PMID: 20510857; PMCID: PMC2894581.
DS Docta Complutense
RD 6 abr 2025