RT Journal Article
T1 Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods
A1 Smith, Peter
A1 Le Devendec, Laëtitia
A1 Jouy, Eric
A1 Larvor, Emeline
A1 Le Breton, Alain
A1 Picón Camacho, Sara
A1 Zrnčić, Snježana
A1 Zupičić, Ivana Giovanna
A1 Oraić, Dražen
A1 Karataş, Süheyla
A1 Verner-Jeffreys, David
A1 Wokorac Joseph, Andrew
A1 Light, Edel
A1 Van Essen-Zandbergen, Alieda
A1 Van Gelderen, Betty
A1 Voorbergen-Laarman, Michal
A1 Haenen, Olga L. M.
A1 Vela Alonso, Ana Isabel
A1 García, María
A1 Baron, Sandrine
AB This work aims to generate the data needed to set epidemiological cut-off values for minimum inhibitory concentration (MIC) and disc-diffusion zone measurements of Vibrio anguillarum. A total of 261 unique isolates were tested, applying standard methods specifying incubation at 28°C for 24-28 h. Aggregated MIC distributions for a total of 247 isolates were determined in 9 laboratories for 11 agents. Data aggregations of the disc zone for the 10 agents analysed contained between 157 and 218 observations made by 4 to 7 laboratories. Acceptable ranges for quality control (QC) reference strains were available for 7 agents and the related multi-laboratory aggregated data were censored, excluding the data of a laboratory that failed to meet QC requirements. Statistical methods were applied to calculate epidemiological cut-off values. Cut-off values for MIC data were calculated for florfenicol (≤1 µg ml-1), gentamicin (≤4 µg ml-1), oxytetracycline (≤0.25 µg ml-1) and trimethoprim/sulfamethoxazole (≤0.125/2.38 µg ml-1). The cut-off values for disc zone data were calculated for enrofloxacin (≥29 mm), florfenicol (≥27 mm), gentamicin (≥19 mm), oxolinic acid (≥24 mm), oxytetracycline (≥24 mm) and trimethoprim/sulfamethoxazole (≥26 mm). MIC and disc-diffusion zone data for the other agents where not supported by QC, thus yielding only provisional cut-off values (meropenem, ceftazidime). Regardless of whether QC is available, some of the aggregated MIC distributions (enrofloxacin, oxolinic acid), disc zone (sulfamethoxazole), and MIC and disc-diffusion distributions (ampicillin, chloramphenicol) did not meet the statistical requirements. The data produced will be submitted to the Clinical Laboratory Standards Institute for their consideration in setting international consensus epidemiological cut-off values.
PB Inter Research Science
SN 0177-5103
YR 2023
FD 2023-08-31
LK https://hdl.handle.net/20.500.14352/104504
UL https://hdl.handle.net/20.500.14352/104504
LA eng
NO Smith P, Le Devendec L, Jouy E, Larvor E et Al. (2023) Epidemiological cut-off values for Vibrio anguillarum MIC and disc diffusion data generated by standardised methods. Dis Aquat Org 155:109-123. https://doi.org/10.3354/dao03745
NO European Commission
NO Ministère de l'Agriculture et de la Souveraineté alimentaire (France)
NO Ministère de la Transition écologique et de la Cohésion des territoires (France)
NO Norwegian Environmental Agency
NO Ministero della Salute (Italia)
DS Docta Complutense
RD 9 abr 2025