RT Journal Article
T1 Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.
A1 Paris, Juan L.
A1 Lafuente Gómez, Nuria
A1 Cabañas Criado, María Victoria
A1 Román Zaragoza, Jesús
A1 Peña López, Juan
A1 Vallet Regí, María Dulce Nombre
AB 3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method. This shaping technology allows the introduction of thermally-labile molecules within the scaffolds during the fabrication procedure. An angiogenic protein, Vascular Endothelial Growth Factor, and an antibiotic, cephalexin, loaded in mesoporous silica nanoparticles, were included to design multifunctional scaffolds for bone reconstruction. The dual release of both molecules showed a marked increase in the number of blood vessels on embryonic day 14 in chicken embryos grown ex ovo, while, at the same time providing an antibiotic local concentration capable of inhibiting Staphylococcus aureus bacterial growth. In this sense, different release patterns, monitored by UV-spectroscopy, could be tailored as a function of the cephalexin loading strategy, either releasing all the loaded cephalexin in the first 4 h or less than 50 % after 24 h. The scaffold surface was characterized by a high hydrophilicity, with contact angles between 50 and 63°, which enabled the adhesion and proliferation of preosteoblastic cells.
PB Elsevier Science BV
SN 1742-7061
YR 2019
FD 2019-03-01
LK https://hdl.handle.net/20.500.14352/13004
UL https://hdl.handle.net/20.500.14352/13004
LA eng
NO Paris, J. L., Lafuente Gómez, N., Cabañas Criado, M. V. et al. «Fabrication of a Nanoparticle-Containing 3D Porous Bone Scaffold with Proangiogenic and Antibacterial Properties». Acta Biomaterialia, vol. 86, marzo de 2019, pp. 441-49. DOI.org (Crossref), https://doi.org/10.1016/j.actbio.2019.01.013.
NO RESEARCHER ID D-9318-2017 (Juan Luis Paris de la Fuente)ORCID 0000-0001-8950-283X (Juan Luis Paris de la Fuente)RESEARCHER ID G-8740-2015 (María Victoria Cabañas Criado)ORCID 0000-0002-4753-5665 (María Victoria Cabañas Criado)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Unión Europea
NO Ministerio de Economía, Comercio y Empresa (España)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 9 abr 2025