RT Journal Article
T1 A Novel Circulating Noncoding Small RNA for the Detection of Acute Myocarditis
A1 Blanco Domínguez, Rafael
A1 Sánchez Díaz, Raquel
A1 Fuente, Hortensia de la
A1 Jiménez Borreguero, Luis J.
A1 Matesanz Marín, Adela
A1 Relaño, Marta
A1 Jiménez Alejandre, Rosa
A1 Linillos Pradillo, Beatriz
A1 Tsilingiri, Katerina
A1 Martín-Mariscal, María L.
A1 Alonso Herranz, Laura
A1 Moreno Muñoz, Guillermo
A1 Martín Asenjo, Roberto
A1 García Guimaraes, Marcos M.
A1 Bruno, Katelyn A.
A1 Dauden, Esteban
A1 González Álvaro, Isidoro
A1 Villar Guimerans, Luisa M.
A1 Martínez León, Amaia
A1 Salvador Garicano. Ane M., 
A1 Michelhaugh, Sam A.
A1 Ibrahim, Nasrien E.
A1 Januzzi, James L.
A1 Kottwitz, Jan
A1 Iliceto, Sabino
A1 Plebani, Mario
A1 Basso, Cristina
A1 Baritussio, Anna
A1 Seguso, Mara
A1 Marcolongo, Renzo
A1 Ricote, Mercedes
A1 Fairweather, DeLisa
A1 Bueno Zamora, Héctor José
A1 Fernández Friera, Leticia
A1 Alfonso Manterola, Fernando
A1 Caforio, Alida L.P.
A1 Pascual Figal, Domingo A.
A1 Heidecker, Bettina
A1 Lüscher, Thomas F.
A1 Das, Saumya
A1 Fuster, Valentín
A1 Ibáñez, Borja
A1 Sánchez Madrid, Francisco
A1 Martín, Pilar
AB The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging (which is not universally available). Additional approaches to diagnosis are desirable. We sought to identify a novel microRNA for the diagnosis of acute myocarditis. METHODS To identify a microRNA specific for myocarditis, we performed microRNA microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells and type 17 helper T (Th17) cells after inducing experimental autoimmune myocarditis or myocardial infarction in mice. We also performed qPCR in samples from coxsackievirus-induced myocarditis in mice. We then identified the human homologue for this microRNA and compared its expression in plasma obtained from patients with acute myocarditis with the expression in various controls. RESULTS We confirmed that Th17 cells, which are characterized by the production of interleukin-17, are a characteristic feature of myocardial injury in the acute phase of myocarditis. The microRNA mmu-miR-721 was synthesized by Th17 cells and was present in the plasma of mice with acute autoimmune or viral myocarditis but not in those with acute myocardial infarction. The human homologue, designated hsa-miR-Chr8:96, was identified in four independent cohorts of patients with myocarditis. The area under the receiver-operating-characteristic curve for this novel microRNA for distinguishing patients with acute myocarditis from those with myocardial infarction was 0.927 (95% confidence interval, 0.879 to 0.975). The microRNA retained its diagnostic value in models after adjustment for age, sex, ejection fraction, and serum troponin level. CONCLUSIONS After identifying a novel microRNA in mice and humans with myocarditis, we found that the human homologue (hsa-miR-Chr8:96) could be used to distinguish patients with myocarditis from those with myocardial infarction.
PB NEJM Group
SN 0028-4793
SN 1533-4406
YR 2021
FD 2021-05-26
LK https://hdl.handle.net/20.500.14352/109742
UL https://hdl.handle.net/20.500.14352/109742
LA eng
NO Blanco-Domínguez R, Sánchez-Díaz R, de la Fuente H, Jiménez-Borreguero LJ, Matesanz-Marín A, Relaño M, et al. A novel circulating microRNA for the detection of acute myocarditis. New England Journal of Medicine. 2021;384(21):2014-27.
DS Docta Complutense
RD 10 abr 2025