RT Journal Article
T1 Resveratrol–cyclodextrin complex affects the expression of genes associated with lipid metabolism in bovine in vitro produced embryos
A1 Torres, Viviana
A1 Hamdi, Meriem
A1 Millán de la Blanca, María Gema
A1 Urrego, R.
A1 Echeverri, J.
A1 López Herrera, A.
A1 Rizos, Dimitri
A1 Gutiérrez Adán, Alfonso
A1 Sánchez Calabuig, María Jesús
AB Antioxidants have been widely used during in vitro production to decrease the negative effect of reactive oxygen species. It was reported that the complex resveratrol-methyl β-cyclodextrin (RV-CD) improves resveratrol's stability and bioavailability and increases its antioxidant activity. This study evaluates the effect of RV-CD during in vitro oocyte maturation (IVM) or in vitro embryo culture (IVC) on developmental competence and quantitative changes in gene expression of developmental important genes. In experiment 1, RV-CD was added to IVM media and maturation level, embryo development and oocytes, cumulus cells, and blastocysts gene expression by RT-qPCR were examined. In experiment 2, presumptive zygotes were cultured in SOF supplemented with RV-CD and embryo development and blastocysts gene expression by RT-qPCR were studied. A group without RV-CD (control- ) and a group with cyclodextrin (control+ ) were included. No differences were found in cleavage rate or blastocyst yield between groups. However, the expression of LIPE was higher in blastocysts derived from oocytes treated with resveratrol compared with control groups (p < .05). Blastocysts produced by IVC with resveratrol showed that RV-CD could modify the expression of genes related to lipid metabolism (CYP51A1, PNPLA2 and MTORC1) compared with control groups (p < .05). RV-CD in the IVM and IVC media could reduce accumulated fat by increasing lipolysis and suppressing lipogenesis of blastocysts.
PB Wiley
SN 0936-6768
YR 2018
FD 2018-03-26
LK https://hdl.handle.net/20.500.14352/106726
UL https://hdl.handle.net/20.500.14352/106726
LA eng
NO Torres V, Hamdi M, Millán de la Blanca MG, et al. Resveratrol–cyclodextrin complex affects the expression of genes associated with lipid metabolism in bovine in vitro produced embryos. Reprod Dom Anim. 2018;53:850–858. https://doi.org/10.1111/rda.13175
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 9 abr 2025