RT Journal Article
T1 Protective actions of cannabidiol on aging-related inflammation, oxidative stress and apoptosis alterations in liver and lung of long evans rats
A1 Rancán, Lisa
A1 Linillos Pradillo, Beatriz
A1 Centeno, Julia
A1 Paredes Royano, Sergio Damián
A1 Vara Ameigeiras, Elena María
A1 Fernández-Tresguerres Hernández, Jesús Ángel
AB Background: Aging is characterised by the progressive accumulation of oxidative damage which leads to inflammation and apoptosis in cells. This affects all tissues in the body causing the deterioration of several organs. Previous studies observed that cannabidiol (CBD) could extend lifespan and health span by its antioxidant, anti-inflammatory and autophagy properties. However, research on the anti-aging effect of CBD is still in the beginning stages. This study aimed to investigate the role of cannabidiol (CBD) in the prevention of age-related alterations in liver and lung using a murine model. Methods: 15-month-old Long Evans rats were treated with 10 mg/kg b.w./day of CBD for 10 weeks and compared to animals of the same age as old control and 2-month-old animals as young control. Gene and/or protein expressions, by RT-qPCR and Western blotting, respectively, were assessed in terms of molecules related to oxidative stress (GST, GPx, GR and HO-1d), inflammation (NFκB, IL-1β and TNF-α) and apoptosis (BAX, Bcl-2, AIF, and CASP-1). In addition, MDA and MPO levels were measured by colorimetric assay. Results were analysed by ANOVA followed by Tukey–Kramer test, considering statistically significant a p < 0.05. Results: GST, GPx and GR expressions were significantly reduced (p < 0.01) in liver samples from old animals compared to young ones and CBD treatment was able to revert it. A significant increase was observed in old animals compared to young ones in relation to oxidative stress markers (MDA and HO-1d), proinflammatory molecules (NFκB, IL-1β and TNF-α), MPO levels and proapoptotic molecules (BAX, AIF and CASP-1), while no significant alterations were observed in the antiapoptotic molecules (Bcl-2). All these changes were more noticeable in the liver, while the lung seemed to be less affected. In almost all the measured parameters, CBD treatment was able to revert the alterations caused by age restoring the levels to those observed in the group of young animals. Conclusions: Chronic treatment with CBD in 15-month-old rats showed beneficial effects in lung and more significantly in liver by reducing the levels of inflammatory, oxidative and apoptotic mediators, and hence the cell damage associated with these three processes inherent to aging.
PB MDPI
SN 2076-3921
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/109257
UL https://hdl.handle.net/20.500.14352/109257
LA eng
NO Antioxidants 2023, 12(10), 1837; https://doi.org/10.3390/antiox12101837
DS Docta Complutense
RD 10 abr 2025