RT Journal Article
T1 Impact of Clopidogrel and Aspirin Treatment on the Expression of Proteins in Platelets from Type-2 Diabetic Patients with Stable Coronary Ischemia
A1 Azcona, Luis
A1 López Farre, Antonio José
A1 Jiménez Mateos Cáceres, Petra
A1 Segura, Antonio
A1 Rodríguez Sierra, Pablo
A1 Modrego, Javier
A1 Zamorano León, José Javier
A1 Macaya Miguel, Carlos
AB The purpose of this study was to compare the effect of dual antiplatelet therapy [clopidogrel + aspirin (ASA)] with respect to ASA on the protein expression of platelets from controlled type-2 diabetic patients with stable coronary ischemia. Patients had been taking ASA (100 mg day) and they were randomized to receive (n = 29) or not (n = 28) 75 mg day clopidogrel for 12 ± 2 weeks in a blind form. Protein expression was analyzed by two-dimensional electrophoresis and mass spectrometry. The protein expression of a limited number of proteins such as actin-binding protein isotypes 2 and 5, lactate dehydrogenase, serotransferrin isotype 4, protein disulfide isomerase-A3 isotype 1, fibrinogen beta chain isotype 5, Ras-related protein Rab-7b isotypes 1 and 6, and immunoglobulin heavy chain was changed after dual antiplatelet therapy. Plasma level of platelet factor 4 (PF4), an in vivo marker of platelet activity, was not different between both groups. These changes suggest lower platelet reactivity after dual antiplatelet therapy in the studied patients. However, the variation in platelet proteome was lower than it would be initially expected, taking into account the apparent clinical beneficial effects of dual antiplatelet therapy. PF4 plasma level was not further decreased in the platelets treated for a longer time than 9-12 months with ASA + clopidogrel, as compared with ASA alone.
PB Elsevier
SN 0022-3549
YR 2012
FD 2012-07-01
LK https://hdl.handle.net/20.500.14352/99363
UL https://hdl.handle.net/20.500.14352/99363
LA eng
NO Azcona L, López Farré AJ, Jiménez Mateos-Cáceres P, Segura A, Rodríguez P, Modrego J, Zamorano-León JJ, Macaya C. Impact of clopidogrel and aspirin treatment on the expression of proteins in platelets from type-2 diabetic patients with stable coronary ischemia. J Pharm Sci. 2012 Aug;101(8):2821-32. doi: 10.1002/jps.23201
NO Fondo de Investigacion de la Seguridad Social
DS Docta Complutense
RD 1 ene 2026