RT Journal Article
T1 Multiplexed magnetic beads-assisted amperometric bioplatforms for global detection of methylations in nucleic acids
A1 Povedano Muñumel, Eloy
A1 Gamella Carballo, María
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Montero-Calle, Ana
A1 Solís-Fernández, Guillermo
A1 Navarro Villoslada, Fernando
A1 Pedrero Muñoz, María
A1 Peláez-García, Alberto
A1 Mendiola, Marta
A1 Hardisson, David
A1 Feliú, Jaime
A1 Barderas Manchado, Rodrigo
A1 Pingarrón Carrazón, José Manuel
AB This work reports the first electrochemical bioplatform developed for the multidetection of 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) in DNA, DNA N6-methyladenine (6mA) and RNA N6-methyladenosine (m6A) methylations at global level. Direct competitive immunoassays were implemented on the surface of magnetic beads (MBs) and optimized for the single amperometric determination of different targets varying in length, sequence and number of methylations on screenprinted carbon electrodes. After evaluating the sensitivity and selectivity of such determinations and the confirmation of no cross-reactivity, a multiplexed disposable platform allowing the simultaneous determination of the mentioned four methylation events in only 45 min has been prepared. The multiplexed bioplatform was successfully applied to the determination of m6A in cellular total RNA and of 5-mC, 5-hmC and 6mA in genomic DNA extracted from tissues. The developed bioplatform showed its usefulness to discriminate the aggressiveness of cancerous cells and between healthy and tumor tissues of colorectal cancer patients.
PB Elsevier
SN 0003-2670
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/91815
UL https://hdl.handle.net/20.500.14352/91815
LA eng
NO Povedano, Eloy, et al. «Multiplexed Magnetic Beads-Assisted Amperometric Bioplatforms for Global Detection of Methylations in Nucleic Acids». Analytica Chimica Acta, vol. 1182, octubre de 2021, p. 338946. https://doi.org/10.1016/j.aca.2021.338946.
DS Docta Complutense
RD 11 abr 2025