RT Journal Article
T1 Circularly polarized luminescence bioimaging using chiral BODIPYs: A model scaffold for advancing unprecedented CPL microscopy using small full-organic probes
A1 Stachelek, Patrycja
A1 Serrano Buitrago, Sergio
A1 Lora Maroto, Beatriz
A1 Pal, Robert
A1 Moya Cerero, Santiago de la
AB Unprecedented circularly polarized luminescence bioimaging (CPL-bioimaging) of live cells using small full-organic probes is first reported. These highly biocompatible and adaptable probes are pivotal to advance emerging CPL Laser-Scanning Confocal Microscopy (CPL-LSCM) as an undeniable tool to distinguish, monitor, and understand the role of chirality in the biological processes. The development of these probes was challenging due to the poor dichroic character associated with the involved CPL emissions. However, the known capability of the BODIPY dyes to be tuned to act as efficient fluorescence bioprobes, together with the capability of the BINOL-O-BODIPY scaffold to enable CPL, allowed the successful design of the first examples of this kind of CPL probes. Interestingly, the developed CPL probes were also multiphoton (MP) active, paving the way for the envisioned MP-CPL-bioimaging. The described full-organic CPL-probe scaffold, based on an optically and biologically tunable BODIPY core, which is chirally perturbed by an enantiopure BINOL moiety, represents, therefore, a simple and readily accessible structural design for advancing efficient CPL probes for bioimaging by CPL-LSCM.
PB ACS
SN 1944-8244
YR 2024
FD 2024-11-25
LK https://hdl.handle.net/20.500.14352/119019
UL https://hdl.handle.net/20.500.14352/119019
LA eng
NO Stachelek, P.; Serrano-Buitrago, S.; Maroto, B. L.; Pal, R.; De La Moya, S. Circularly Polarized Luminescence Bioimaging Using Chiral BODIPYs: A Model Scaffold for Advancing Unprecedented CPL Microscopy Using Small Full-Organic Probes. ACS Appl. Mater. Interfaces 2024, 16 (49), 67246–67254. https://doi.org/10.1021/acsami.4c14127.
NO R.P. acknowledges support from the Royal Society University Research Fellowship URF\R\191002, H2020-MSCA-ITN-859752 HEL4CHIROLED, BBSRC BB/S017615/1 and BB/X001172/1, and EPSRC EP/X040259/1. P.S. acknowledges support from the Royal Society University Research Fellowship URF\R1\221121. S.d.l.M., B.L.M., and S.S.-B. acknowledge support from the Spanish Ministerio de Ciencia e Innovación (MICINN, AEI/10.13039/501100011033) PID2020-114755GB-C32. S.S.-B. acknowledges the Universidad Complutense de Madrid (UCM) for a predoctoral contract (CT58/21-CT59/21).
NO Royal Society University Research
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 3 abr 2025