RT Journal Article
T1 Serum MicroRNAs as Biomarkers of Sepsis and Resuscitation
A1 Oteiza, Lorena
A1 Ferruelo, Antonio
A1 Nín, Nicolás
A1 Arenillas Baquero, Mario
A1 Paula, Marta de
A1 Pandolfi, Rachele
A1 Moreno Gutiérrez, Laura
A1 Herrero, Raquel
A1 González Rodríguez, Paloma
A1 Peñuelas, Óscar
A1 Pérez Vizcaíno, Francisco
A1 Lorente, José Ángel
AB There is a lack of biomarkers of sepsis and the resuscitation status. Our objective was to prove that the serum expression of certain microribonucleic acids (miRNAs) is differentially regulated in sepsis and is sensitive to different resuscitation regimes. Anesthetized pigs (Sus scrofa domesticus) received no treatment (n = 15) or intravenous live E. coli (n = 24). The septic animals received 0.9% saline at 4 mL/kg/h (n = 8) (low resuscitation group (LoR)) or 10–17 mL/kg/h (high resuscitation group (HiR)) (n = 8 each group). Blood samples were obtained at the end of the experiment for measurement of seven different miRNAs (RT-qPCR, Qiagen, Hilden, Germany). The serum expression of miR-146a-5p and miR-34a-5p increased significantly in the septic group, and miR-146a-5p was significantly lower in the HiR group than in the LoR group. The toll-like receptor signaling pathway involving 22 target proteins was significantly (adjusted p = 3.87 × 10−4) regulated by these two microRNAs (KEGG). Highly significant (p value = 2.22 × 10−16) protein–protein interactions (STRING) were revealed for these 22 hits. MiR-146a-5p and miR-34a-5p were identified as biomarkers of sepsis, and miRNA146a-5p seemed to be a biomarker of the intensity of the resuscitation
PB MDPI
SN 2076-3417
YR 2021
FD 2021-12-06
LK https://hdl.handle.net/20.500.14352/5068
UL https://hdl.handle.net/20.500.14352/5068
LA eng
DS Docta Complutense
RD 9 abr 2025