RT Journal Article
T1 VIP impairs acquisition of the macrophage proinflammatory polarization profile
A1 Carrión Caballo, Mar
A1 Pérez García, Selene
A1 Martínez Mora, María Del Carmen
A1 Juarranz Moratilla, Yasmina
A1 Estrada Capetillo, Lizbeth
A1 Puig-Kröger, Amaya
A1 Pérez Gomáriz, Rosa María
A1 Gutiérrez Cañas, Irene
AB This study tested the hypothesis that vasoactive intestinal peptide (VIP) is able to modify the macrophage inflammatory profile, thus supporting its therapeutic role in autoimmune diseases. Macrophages are innate immune cells that display a variety of functions and inflammatory profiles in response to the environment that critically controls their polarization.Deregulation between the pro- and anti-inflammatory phenotypes has been involved in different pathologies.Rheumatoid arthritis (RA) is an autoimmune disease, in which macrophages are considered central effectors of synovial inflammation, displaying a proinflammatory profile.VIP is a pleiotropic neuropeptide with proven antiinflammatory actions. As modulation of the macrophage phenotype has been implicated in the resolution of inflammatory diseases, we evaluated whether VIP is able to modulate human macrophage polarization. In vitropolarized macrophages by GM-CSF (GM-MØ), with a proinflammatory profile, expressed higher levels of VIP receptors, vasoactive intestinal polypeptide receptors 1 and 2 (VPAC1 and VPAC2, respectively), than macrophages polarized by M-CSF (M-MØ) with anti-inflammatory activities. RA synovial macrophages, according to their GM-CSF-like polarization state, expressed both VPAC1 and VPAC2. In vitro-generated GM-MØ exposed to VIP exhibited an up-regulation of M-MØ gene marker expression, whereas their proinflammatory cytokine profile was reduced in favor of an anti-inflammatory function. Likewise, in GM-MØ, generated in the presence of VIP, VIP somehow changes the macrophages physiology profile to a less-damaging phenotype. Therefore, these results add new value to VIP as an immunomodulatory agent on inflammatory diseases.
SN 0741-5400
YR 2016
FD 2016-12
LK https://hdl.handle.net/20.500.14352/17628
UL https://hdl.handle.net/20.500.14352/17628
LA eng
NO Carrión Caballo, M., Pérez García, S., Martínez Mora, M. C. et al. «VIP Impairs Acquisition of the Macrophage Proinflammatory Polarization Profile». Journal of Leukocyte Biology, vol. 100, n.o 6, diciembre de 2016, pp. 1385-93. Crossref, https://doi.org/10.1189/jlb.3a0116-032rr.
NO Comunidad de Madrid/Fondo Europeo de Desarrollo Regional
NO Instituto de Salud Carlos III
NO Federación Española de Enfermedades Raras
NO Redes Temáticas de Investigación Cooperativa
NO Red de Investigación en Inflamación y Enfermedades Reumáticas
DS Docta Complutense
RD 7 abr 2025