RT Journal Article
T1 One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry
A1 Acosta, Javier
A1 Del Arco, Jon
A1 Martínez Pascual, Sara
A1 Clemente Suárez, Vicente Javier
A1 Fernández Lucas, Jesús
AB Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic compounds. Natural metabolic pathways usually involve complex networks to support cellular growth and survival. In this regard, multi-enzymatic systems are valuable tools for the production of a wide variety of organic compounds. Methods: The production of different purine nucleosides and nucleoside-5′-monophosphates has been performed for first time, catalyzed by the sequential action of 2′-deoxyribosyltransferase from Lactobacillus delbrueckii (LdNDT) and hypoxanthine-guanine-xanthine phosphoribosyltransferase from Thermus themophilus HB8 (TtHGXPRT). Results: The biochemical characterization of LdNDT reveals that the enzyme is active and stable in a broad range of pH, temperature, and ionic strength. Substrate specificity studies showed a high promiscuity in the recognition of purine analogues. Finally, the enzymatic production of different purine derivatives was performed to evaluate the efficiency of multi-enzymatic system LdNDT/TtHGXPRT. Conclusions: The production of different therapeutic purine nucleosides was efficiently catalyzed by LdNDT/TtHGXPRT. In addition, the resulting by-products were converted to IMP and GMP. Taking all of these features, this bioprocess entails an efficient, sustainable, and economical alternative to chemical synthetic methods.
PB MDPI
SN 2073-4344
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/110367
UL https://hdl.handle.net/20.500.14352/110367
LA eng
NO Acosta, J., Del Arco, J., Martinez-Pascual, S., Clemente-Suárez, V. J., & Fernández-Lucas, J. (2018). One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry. Catalysts, 8(1). https://doi.org/10.3390/CATAL8010009
NO Fundación Santander
NO Universidad Europea de Madrid
DS Docta Complutense
RD 17 abr 2025