%0 Journal Article
%A López Gordillo, Yamila
%A Maldonado Bautista, Estela
%A Nogales, Laura
%A Río Sevilla, Aurora Del
%A Barrio Asensio, María Del Carmen
%A Murillo González, Jorge Alfonso
%A Martínez Sanz, Elena
%A Paradas Lara, Irene
%A Alonso Revuelta, María Isabel
%A Partearroyo, Teresa
%A Martínez Álvarez, María Concepción
%T Maternal folic acid supplementation reduces the severity of cleft palate in Tgf-β3 null mutant mice
%D 2019
%@ 0031-3998
%U https://hdl.handle.net/20.500.14352/93982
%X BACKGROUND: Cleft palate (CP) constitutes the most frequently seen orofacial cleft and is often associated with low folate status.Folate plays an essential role in the human body as a major coenzyme in one-carbon metabolism, including DNA synthesis, repair,and methylation. Whether the administration of isolated folic acid (FA) supplements prevents the CP caused by genetic mutations isunknown, as is its effect on the mechanisms leading to palate fusion.METHODS: FA was administered to females from two different strains of transforming growth factor β3 heterozygous mice. Nullmutant progeny of these mice exhibit CP in 100% of cases of varying severity. We measured cleft length, height of palatal shelfadhesion, and the number of proliferating mesenchymal cells. Immunohistochemistry was also carried for collagen IV, laminin,fibronectin, cytokeratin-17, and EGF.RESULTS: FA supplementation significantly reduced CP severity and improved palatal shelf adhesion in both strains both in vivoand in vitro. Medial edge epithelium proliferation increased, and its differentiation was normalized as indicated by the presenceand disposition of collagen IV, laminin, fibronectin, and cytokeratin-17.CONCLUSIONS: A maternal FA supplementation reduces the CP appearance by improving the mechanisms leading to palatal shelfadhesion.
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