RT Journal Article
T1 Maternal folic acid supplementation reduces the severity of cleft palate in Tgf-β3 null mutant mice
A1 López Gordillo, Yamila
A1 Maldonado Bautista, Estela
A1 Nogales, Laura
A1 Río Sevilla, Aurora Del
A1 Barrio Asensio, María Del Carmen
A1 Murillo González, Jorge Alfonso
A1 Martínez Sanz, Elena
A1 Paradas Lara, Irene
A1 Alonso Revuelta, María Isabel
A1 Partearroyo, Teresa
A1 Martínez Álvarez, María Concepción
AB BACKGROUND: Cleft palate (CP) constitutes the most frequently seen orofacial cleft and is often associated with low folate status.Folate plays an essential role in the human body as a major coenzyme in one-carbon metabolism, including DNA synthesis, repair,and methylation. Whether the administration of isolated folic acid (FA) supplements prevents the CP caused by genetic mutations isunknown, as is its effect on the mechanisms leading to palate fusion.METHODS: FA was administered to females from two different strains of transforming growth factor β3 heterozygous mice. Nullmutant progeny of these mice exhibit CP in 100% of cases of varying severity. We measured cleft length, height of palatal shelfadhesion, and the number of proliferating mesenchymal cells. Immunohistochemistry was also carried for collagen IV, laminin,fibronectin, cytokeratin-17, and EGF.RESULTS: FA supplementation significantly reduced CP severity and improved palatal shelf adhesion in both strains both in vivoand in vitro. Medial edge epithelium proliferation increased, and its differentiation was normalized as indicated by the presenceand disposition of collagen IV, laminin, fibronectin, and cytokeratin-17.CONCLUSIONS: A maternal FA supplementation reduces the CP appearance by improving the mechanisms leading to palatal shelfadhesion.
PB SPRINGERNATURE
SN 0031-3998
YR 2019
FD 2019-01-15
LK https://hdl.handle.net/20.500.14352/93982
UL https://hdl.handle.net/20.500.14352/93982
LA eng
NO A, Barrio MC, Murillo J, Martínez-Sanz E, Paradas-Lara I, Alonso MI, Partearroyo T, Martínez-Álvarez C. Maternal folic acid supplementation reduces the severity of cleft palate in Tgf-β3 null mutant mice. Pediatr Res. 2019 Mar;85(4):566-573.
NO Ministerio de Economía y Competitividad de España
NO Banco Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 20 ago 2024