RT Journal Article
T1 Improvement of Enzyme Properties with a Two-Step Immobilizaton Process on Novel Heterofunctional Supports
A1 Mateo, Cesar
A1 Bolívar Bolívar, Juan Manuel
A1 Godoy, Cesar
A1 Rocha Martín, Javier
A1 Pessela, Benevides
A1 Curiel, José Antonio
A1 Muñoz, Rosario
A1 Guisan, Jose
A1 Fernández-Lorente, Gloria
AB Novel heterofunctional glyoxyl-agarose supports were prepared. These supports contain a high concentration of groups (such as quaternary ammonium groups, carboxyl groups, and metal chelates) that are capable of adsorbing proteins, physically or chemically, at neutral pH as well as a high concentration of glyoxyl groups that are unable to immobilize covalently proteins at neutral pH. By using these supports, a two-step immobilization protocol was developed. In the first step, enzymes were adsorbed at pH 7.0 through adsorption of surface regions, which are complementary to the adsorbing groups on the support, and in the second step, the immobilized derivatives were incubated under alkaline conditions to promote an intramolecular multipoint covalent attachment between the glyoxyl groups on the support and the amino groups on the enzyme surface. These new derivatives were compared with those obtained on a monofunctional glyoxyl support at pH 10, in which the region with the greatest number of lysine residues participates in the first immobilization step. In some cases, multipoint immobilization on heterofunctional supports was much more efficient than what was achieved on the monofunctional support. For example, derivatives of tannase from Lactobacillus plantarum on an amino-glyoxyl heterofunctional support were 20-fold more stable than the best derivative on a monofunctional glyoxyl support. Derivatives of lipase from Geobacillus thermocatenulatus (BTL2) on the amino-glyoxyl supports were two times more active and four times more enantioselective than the corresponding monofunctional glyoxyl support derivative.
PB American Chemical Society
SN 1525-7797
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/92045
UL https://hdl.handle.net/20.500.14352/92045
LA eng
NO Mateo, Cesar, et al. «Improvement of Enzyme Properties with a Two-Step Immobilizaton Process on Novel Heterofunctional Supports». Biomacromolecules, vol. 11, n.o 11, noviembre de 2010, pp. 3112-17. https://doi.org/10.1021/bm100916r.
NO Ministerio de Ciencia e Innovación (España)
NO Instituto de Salud Carlos III
NO Comunidad de Madrid
DS Docta Complutense
RD 11 abr 2025