RT Journal Article
T1 Immunohistochemical, histomorphometric, and gingival crevicular fluid analysis of residual and shallow periodontal pockets in patients with periodontitis Stages III and IV
A1 Martínez Villa, Sergio
A1 Sanz Martín, Ignacio
A1 Maldonado Bautista, Estela
A1 Virto Ruiz, Leire
A1 Sanz Esporrín, Javier
A1 Sanz Alonso, Mariano
AB Background: To study the differences between shallow and residual periodontal pockets in patients with periodontitis (Stages III and IV) after non-surgical periodontal treatment.Methods: Twenty patients diagnosed of periodontitis who were scheduled for periodontal surgery were included. In each patient, a palatal shallow site (≤3 mm) and a residual site (≥5 mm) were selected and GCF samples were processed by Luminex® analysis to determine the concentrations of interleukins (IL-1β, IL-6, IL-10, and IL-17a). During the periodontal surgery gingival biopsies were collected and processed for histo-morphometric and immunohistochemical evaluation to determine the extent of connective tissue inflammatory infiltrate (CTII) using the following markers (CD4, CD5, CD8, CD14, CD19, Elastase, and Syndecan). Mean differences between shallow and residual pockets samples, as well as correlations between GCF cytokine concentrations, area of CTII, and cellularity of the CTII were calculated.Results: A total of 15 patients were finally included, with analysis of 30 histological specimens and 30 GCF samples. Residual pockets presented significantly higher mean GCF volume, higher mean area of CTII and higher concentrations of IL-1β and IL-6 in GCF than shallow pockets. A significant correlation was detected between IL-10 levels and the CTII area, IL-10 and the percentage of Syndecan, and the area of CTII and the percentages of CD14 and Syndecan.Conclusions: The concentration of GCF cytokines did not correlate with the area of CTII measured histologically. A residual CTII and elevated concentrations of proinflammatory cytokines and cells were present in all sites 2 months after non-surgical treatment. The lack of healthy controls does not allow to establish differences between both groups.
PB The American Academy of Periodontology
SN 0022-3492
YR 2019
FD 2019-12-20
LK https://hdl.handle.net/20.500.14352/94139
UL https://hdl.handle.net/20.500.14352/94139
LA eng
NO Martínez-Villa S, Sanz-Martín I, Maldonado E, Virto L, Sanz-Esporrín J, Sanz M. Immunohistochemical, histomorphometric, and gingival crevicular fluid analysis of residual and shallow periodontal pockets in patients with periodontitis Stages III and IV. J Periodontol. 2020 Jul;91(7):870-879. doi: 10.1002/JPER.19-0035. Epub 2020 Jan 16
NO Issue Online: 15 July 2020Version of Record online: 16 January 2020Accepted manuscript online: 20 December 2019Manuscript accepted: 23 September 2019Manuscript revised: 07 August 2019Manuscript received: 21 January 2019
NO Universidad Complutense de Madrid (España)
DS Docta Complutense
RD 20 ago 2024