RT Journal Article
T1 Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
A1 Maestro García-Donas, María Beatriz
A1 Velasco, I.
A1 Castillejo, I.
A1 Arevalo-Rodriguez, M.
A1 Cebolla, A.
A1 Sanz, J.M.
AB We present a novel procedure for affinity partitioning of recombinant proteins fused to the choline-binding module C-LytA in aqueous two-phase systems containing poly(ethylene glycol) (PEG). Proteins tagged with the C-LytA module and exposed to the two-phase systems are quantitatively localized in the PEG-rich phase, whereas subsequent addition of the natural ligand choline specifically shifts their localization to the PEG-poor phase by displacement of the polymer from the binding sites. The described procedure is simple, scalable and reproducible, and has been successfully applied to the purification of four diverse proteins, resulting in high yields and purity.
PB Elsevier
SN 0021-9673
YR 2008
FD 2008-09-03
LK https://hdl.handle.net/20.500.14352/93429
UL https://hdl.handle.net/20.500.14352/93429
LA eng
NO Ministerio de Educación y Ciencia (MEC)
DS Docta Complutense
RD 9 abr 2025