RT Journal Article T1 Welfare of horses during killing for purposes other than slaughter A1 Nielsen, Søren Saxmose A1 Álvarez Sánchez, Julio A1 Boklund, Anette A1 Dippel, Sabine A1 Dorea, Fernanda A1 Figuerola, Jordi A1 Herskin, Mette A1 Miranda Chueca, Miguel Angel A1 Nannoni, Eleonora A1 Nonno, Romolo A1 Riber, Anja A1 Stahl, Karl A1 Stegeman, Jan Arend A1 Thulke, Hans-Hermann A1 Tuyttens, Frank A1 Winckler, Christoph A1 Raj, Mohan A1 Velarde, Antonio A1 Candiani, Denise A1 Van der Stede, Yves A1 Michel, Virginie AB Horses of different ages may have to be killed on-farm for purposes other thanslaughter (where slaughter is defined as killing for human consumption) either in-dividually (i.e. on-farm killing of unproductive, injured or terminally ill animals) oron a large-scale (i.e. depopulation for disease control purposes and other situa-tions, such as environmental contamination, disaster management, etc.). The pur-pose of this opinion is to assess the hazards and welfare consequences associatedwith the on-farm killing of horses. The killing procedure is divided into Phase 1(pre-killing), which includes the processes (i) handling and moving the animals tothe killing place and (ii) restraint of the animals before application of the killingmethod; and Phase 2 (stunning and/or killing), which includes stunning and kill-ing of the animals (for methods that require one step for stunning and another forsubsequent killing) or killing only (for methods that simultaneously stun and killthe animals). Three stunning and/or killing methods for Phase 2 for horses wereidentified: (i) penetrative captive bolt followed by killing, (ii) firearms with free pro-jectiles and (iii) lethal injection. Welfare consequences that horses may experienceduring each process (e.g. handling stress, restriction of movement and injuries dur-ing restraint) were identified and potential hazards are listed for all phases, alongwith preventive and corrective measures. Animal-based measures (ABMs) to assessall identified welfare consequences were proposed. During the application of thestunning and/or killing methods, horses will experience pain and fear if they areineffectively stunned/killed or if they recover consciousness. A flowchart includ-ing ABMs for the assessment of consciousness and death to monitor stunning andkilling effectiveness is provided. Additionally, specific practices deemed unaccep-table on welfare grounds are listed PB Wiley SN 1831-4732 YR 2025 FD 2025 LK https://hdl.handle.net/20.500.14352/118227 UL https://hdl.handle.net/20.500.14352/118227 LA eng NO Nielsen, S. S., Alvarez, J., Boklund, A., Dippel, S., Dorea, F., Figuerola, J., Herskin, M., Miranda Chueca, M. A., Nannoni, E., Nonno, R., Riber, A., Stahl, K., Stegeman, J. A., Thulke, H.-H., Tuyttens, F., Winckler, C., Raj, M., Velarde, A., … Michel, V. (2025). Welfare of horses during killing for purposes other than slaughter. EFSA Journal, 23(1), e9195. https://doi.org/10.2903/j.efsa.2025.9195 NO European Commission DS Docta Complutense RD 6 abr 2025