RT Journal Article
T1 2′-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
A1 Fresco Taboada, Alba
A1 Fernández Lucas, Jesús
A1 Acebal Sarabia, Carmen
A1 Arroyo Sánchez, Miguel
A1 Ramón, Fernando
A1 De La Mata Riesco, Mª Isabel
A1 Mancheño, José
AB Structure-function relationships of a novel 20-deoxyribosyltransferase from the psychrotolerant bacterium Bacillus psychrosaccharolyticus (BpNDT) have been exhaustively studied by biochemical and high resolution crystallographic analyses. Despite BpNDT exhibiting some structural features characteristic of cold-adapted enzymes such as localized flexibility in critical loops, its biochemical properties are typical of mesophilic enzymes. BpNDT is a highly symmetrical homohexamer with tightly associated subunits that possesses flexible and short loops bordering the active sites. The catalytic center is essentially identical to that of other mesophilic homologues. Moreover, BpNDT shows that it is a mesophilic-like enzyme since it is not heat-labile and exhibits an apparent unfolding temperature (Tm) of 49 ◦C, being active during 96 h at 40 and 50 ◦C. Finally, BpNDT synthesizes natural and modified nucleosides, with preference for purines as acceptors and pyrimidine nucleosides as donors. Remarkably, the synthesis of several therapeutic nucleosides has been efficiently carried out. In this sense, 5-hydroxymethyl-20 -deoxyuridine (5-HMdUrd), 7-deaza-6-hydroxypurine-20 -deoxyriboside (7-DHPdRib) and theophylline-20 -deoxyriboside were synthesized for the first time by an NDT enzyme, showing the biotechnological interest of BpNDT.
PB MDPI
SN 2073-4344
YR 2018
FD 2018-01-03
LK https://hdl.handle.net/20.500.14352/12486
UL https://hdl.handle.net/20.500.14352/12486
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Comunidad de Madrid
DS Docta Complutense
RD 6 abr 2025