RT Journal Article
T1 Reference intervals for reticulocyte count and derived reticulocyte parameters in a cohort of healthy adults
A1 Posada Franco, Yolanda
A1 García‐Álvarez, Ana
A1 Hernández‐Álvarez, Elena
A1 Serrano‐García, Irene
A1 Contera‐Raposo, Rocío
A1 Martínez‐Novillo González, Mercedes
A1 Sanz‐Casla, María Teresa
A1 Martínez-Novillo González, Mercedes
AB IntroductionReticulocyte count and novel derived parameters provide insight into the effectiveness of erythropoiesis and may be useful tools in the classification and diagnosis of anemias. However, there is no standardisation, so we consider it necessary that each laboratory evaluates the parameters according to its own methodology and instrumentation and establishes its own reference ranges. Our aim was to establish the reference intervals (RIs) of reticulocyte profile provided by the Beckman Coulter DxH 900 haematological autoanalyzer in our reference population.MethodsOne hundred and seventy‐five healthy adults (18 to 62 years) were included. Subjects were collected from the blood donation centre of the Hospital Clínico San Carlos (Madrid, Spain) upon informed consent. Whole blood was collected and assayed for 14 haematological parameters on the Beckman Coulter DxH 900 analyzer in the haematology laboratory of the Clinical Analysis Department. RIs were established as per Clinical and Laboratory Standards Institute EP28‐A3c guidelines using three different statistical approaches.ResultsRIs estimated using the non‐parametric method and the Harrell‐Davis bootstrap method were very similar. RIs estimated by the robust method were narrower. Gender partitioning was required for two haematological parameters (low haemoglobin density (LHD) and microcytic anaemia factor (MAF)). The rest of the parameters did not need to be partitioned according to Lahti's method.ConclusionRIs have been established for 14 hematologic parameters of the reticulocyte profile for the Beckman Coulter DxH 900 haematology analyzer using a healthy cohort of adult subjects.
PB Wiley
SN 1751-5521
YR 2024
FD 2024-06-25
LK https://hdl.handle.net/20.500.14352/118140
UL https://hdl.handle.net/20.500.14352/118140
LA eng
NO Posada‐Franco, Y., García‐Álvarez, A., Hernández‐Álvarez, E., Serrano‐García, I., Contera‐Raposo, R., Martínez‐Novillo González, M., & Sanz‐Casla, M. T. (2024). Reference intervals for reticulocyte count and derived reticulocyte parameters in a cohort of healthy adults. International Journal of Laboratory Hematology, 46(6), 997-1003. https://doi.org/10.1111/ijlh.14332
DS Docta Complutense
RD 5 abr 2025