RT Journal Article
T1 Characterization of Relevant Biomarkers for the Diagnosis of Food Allergies: An Overview of the 2S Albumin Family
A1 Bueno Díaz, Cristina
A1 Martín-Pedraza, Laura
A1 Parrón Ballesteros, Jorge
A1 Cuesta-Herranz, Javier
A1 Cabanillas, Beatriz
A1 Pastor Vargas, Carlos
A1 Batanero Cremades, Eva
A1 Villalba Díaz, María Teresa
AB 2S albumins are relevant and often major allergens from several tree nuts and seeds, affecting mainly children and young people. The present study aims to assess how the structural features of 2S albumins could affect their immunogenic capacity, which is essential to comprehend the role of these proteins in food allergy. For this purpose, twelve 2S albumins were isolated from their respective extracts by chromatographic methods and identified by MALDI-TOF massspectrometry. Their molecular and structural characterization was conducted by electrophoretic, spectroscopic and in silico methods, showing that these are small proteins that comprise a wide range of isoelectric points, displaying a general high structure stability to thermal treatment. Despite low amino acid sequence identity, these proteins share structural features, pointing conformational epitopes to explain cross-reactivity between them. Immunoblotting with allergic patients’ sera revealed those possible correlations between evolutionarily distant 2S albumins from different sources. The availability of a well-characterized panel of 2S albumins from plant-derived sources allowed establishing correlations between their structural features and their allergenic potential, including their role in cross-reactivity processes
PB MDPI
SN 2304-8158
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/93354
UL https://hdl.handle.net/20.500.14352/93354
LA eng
NO Bueno-Díaz, C.; Martín-Pedraza, L.; Parrón, J.; Cuesta-Herranz, J.; Cabanillas, B.; Pastor-Vargas, C.; Batanero, E.; Villalba, M. Characterization of Relevant Biomarkers for the Diagnosis of Food Allergies: An Overview of the 2S Albumin Family. Foods 2021, 10, 1235. https://doi.org/10.3390/foods10061235
NO Ministerio de Economia y ́Competitividad (España)
NO European Commission
NO Ministerio de Educación (España)
DS Docta Complutense
RD 11 abr 2025