RT Journal Article
T1 Assessment of myocardial viscoelasticity with Brillouin spectroscopy in myocardial infarction and aortic stenosis models
A1 Villalba Orero, María
A1 Jiménez-Riobóo, Rafael J
A1 Gontán, Nuria
A1 Sanderson, Daniel
A1 López-Olañeta, Marina
A1 García-Pavía, Pablo
A1 Desco, Manuel
A1 Lara-Pezzi, Enrique
A1 Gómez-Gaviro, Maria Victoria
AB Heart diseases are associated with changes in the biomechanical properties of the myocardial wall. However, there is no modality available to assess myocardial stiffness directly. Brillouin microspectroscopy (mBS) is a consolidated mechanical characterization technique, applied to the study of the viscoelastic and elastic behavior of biological samples and may be a valuable tool for assessing the viscoelastic properties of the cardiac tissue. In this work, viscosity and elasticity were assessed using mBS in heart samples obtained from healthy and unhealthy mice (n = 6 per group). Speckle-tracking echocardiography (STE) was performed to evaluate heart deformation. We found that mBS was able to detect changes in stiffness in the ventricles in healthy myocardium. The right ventricle showed reduced stiffness, in agreement with its increased compliance. mBS measurements correlated strongly with STE data, highlighting the association between displacement and stiffness in myocardial regions. This correlation was lost in pathological conditions studied. The scar region in the infarcted heart presented changes in stiffness when compared to the rest of the heart, and the hypertrophied left ventricle showed increased stiffness following aortic stenosis, compared to the right ventricle. We demonstrate that mBS can be applied to determine myocardial stiffness, that measurements correlate with functional parameters and that they change with disease.
PB Nature Publishing Group
SN 2045-2322
YR 2021
FD 2021-11-01
LK https://hdl.handle.net/20.500.14352/95855
UL https://hdl.handle.net/20.500.14352/95855
LA eng
NO Villalba-Orero, M., Jiménez-Riobóo, R. J., Gontán, N., Sanderson, D., López-Olañeta, M., García-Pavía, P., Desco, M., Lara-Pezzi, E., & Gómez-Gaviro, M. V. (2021). Assessment of myocardial viscoelasticity with Brillouin spectroscopy in myocardial infarction and aortic stenosis models. Scientific reports, 11(1), 21369. https://doi.org/10.1038/s41598-021-00661-4
NO This study has been funded by Instituto de Salud Carlos III through the project PI18/00462 to M.V.G.G., co‐funded by European Regional Development Fund “A way to make Europe”, (CB16/11/00432 to P.G.-P. and E.L-P, and RD12/0042/005) and the Spanish Ministerio de Ciencia (RTI2018-096961-B-I00 to E.L-P. and RTI2018-096918-B-C41 to R.J.J.R.). Tis study was also supported by the Plan Estatal de I+D+I 2013-2016, with funding from the European Regional Development Fund (ERDF) “A way to build Europe” initiative. Te CNIC is supported by Instituto de Salud Carlos III (ISCIII), Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505).
NO Instituto de Salud Carlos III
NO Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
NO Ministerio de Ciencia. (España)
NO Ministerio de Ciencia e Innovación (España)
NO Fundación ProCNIC
NO Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
DS Docta Complutense
RD 7 oct 2024