RT Journal Article
T1 Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A in lactic acid bacteria
A1 Borrero Del Pino, Juan
A1 Jiménez Martínez, Juan J.
A1 Gútiez Sainz-Pardo, Loreto
A1 Herranz Benito, Carmen
A1 Cintas Izarra, Luis Miguel
A1 Hernández Cruza, Pablo Elpidio
AB Replacement of the leader sequence (LS) of the bacteriocin enterocin A (LS entA) by the signal peptides (SP) of the protein Usp45 (SP usp45), and the bacteriocins enterocin P (SP entP), and hiracin JM79 (SP hirJM79) permits the production, secretion, and functional expression of EntA by different lactic acid bacteria (LAB). Chimeric genes encoding the SP usp45, the SP entP, and the SP hirJM79 fused to mature EntA plus the EntA immunity genes (entA+entiA) were cloned into the expression vectors pNZ8048 and pMSP3545, under control of the inducible P nisA promoter, and in pMG36c, under control of the constitutive P 32 promoter. The amount, antimicrobial activity, and specific antimicrobial activity of the EntA produced by the recombinant Lactococcus lactis, Enterococcus faecium, E. faecalis, Lactobacillus sakei and Pediococcus acidilactici hosts varied depending on the signal peptide, the expression vector, and the host strain. However, the antimicrobial activity and the specific antimicrobial activity of the EntA produced by most of the LAB transformants was lower than expected from their production. The supernatants of the recombinant L. lactis NZ9000 (pNZUAI) and L. lactis NZ9000 (pNZHAI), overproducers of EntA, showed a 1.2- to 5.1-fold higher antimicrobial activity than that of the natural producer E. faecium T136 against different Listeria spp.
PB Elsevier
SN 0168-1656
YR 2011
FD 2011-08-04
LK https://hdl.handle.net/20.500.14352/115198
UL https://hdl.handle.net/20.500.14352/115198
LA eng
NO Juan Borrero, Juan J. Jiménez, Loreto Gútiez, Carmen Herranz, Luis M. Cintas, Pablo E. Hernández, Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A in lactic acid bacteria, Journal of Biotechnology, Volume 156, Issue 1, 2011, Pages 76-86, https://doi.org/10.1016/j.jbiotec.2011.07.038.
NO Ministerio de Educación y Ciencia (España)
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 19 abr 2025