RT Journal Article
T1 Identification of thaumatin‐like protein and aspartyl protease as new major allergens in lettuce (Lactuca sativa).
A1 Muñoz‐García, Esther
A1 Luengo‐Sánchez, Olga
A1 Haroun‐Díaz, Elisa
A1 Sanz Maroto, Aroa
A1 Palacín, Arancha
A1 Díaz –Perales, Araceli
A1 de las Heras Gozalo, Manuel
A1 Labrador‐Horrillo, Moisés
A1 Vivanco Martínez, Fernando
A1 Cuesta‐Herranz, Javier
A1 Pastor Vargas, Carlos
AB Scope: Today, about 2-8% of the population of Western countries exhibits some type of food allergy whose impact ranges from localized symptoms confined to the oral mucosa to severe anaphylactic reactions. Consumed worldwide, lettuce is a Compositae family vegetable that can elicit allergic reactions. To date, however, only one lipid transfer protein has been described in allergic reaction to lettuce. The aim of this study was to identify potential new allergens involved in lettuce allergy.Methods and results: Sera from 42 Spanish lettuce-allergic patients were obtained from patients recruited at the outpatient clinic. IgE-binding proteins were detected by SDS-PAGE and immunoblotting. Molecular characterization of IgE-binding bands was performed by MS. Thaumatin was purified using the Agilent 3100 OFFGEL system. The IgE-binding bands recognized in the sera of more than 50% of patients were identified as lipid transfer protein (9 kDa), a thaumatin-like protein (26 kDa), and an aspartyl protease (35 and 45 kDa). ELISA inhibition studies were performed to confirm the IgE reactivity of the purified allergen.Conclusion: Two new major lettuce allergens-a thaumatin-like protein and an aspartyl protease-have been identified and characterized. These allergens may be used to improve both diagnosis and treatment of lettuce-allergic patients.
PB Wiley
SN 1613-4125
SN 1613-4133
YR 2013
FD 2013-08-25
LK https://hdl.handle.net/20.500.14352/107711
UL https://hdl.handle.net/20.500.14352/107711
LA eng
NO Muñoz-García E, Luengo-Sánchez O, Haroun-Díaz E, Maroto AS, Palacín A, Díaz-Perales A, de las Heras Gozalo M, Labrador-Horrillo M, Vivanco F, Cuesta-Herranz J, Pastor-Vargas C. Identification of thaumatin-like protein and aspartyl protease as new major allergens in lettuce (Lactuca sativa). Mol Nutr Food Res. 2013 Dec;57(12):2245-52. doi: 10.1002/mnfr.201300139. Epub 2013 Aug 25. PMID: 23983075.
DS Docta Complutense
RD 6 oct 2024