RT Journal Article
T1 Prognostic value of telomere function in gastric cancers with and without microsatellite instability
A1 Pascua García, Irene
A1 Fernández Marcelo, Tamara
A1 Sánchez Pernaute, Andrés
A1 Juan, Carmen de
A1 Head, Jacqueline
A1 Torres García, Antonio José
A1 Iniesta, Pilar
AB Objective To identify molecular markers that may be useful in the selection of gastric cancer patients with different prognoses, we investigated telomere function in gastric cancers with and without microsatellite instability (MSI).Materials and methods We analyzed 83 gastric cancers and its paired-normal tissues to investigate MSI and telomere function. MSI was established using five polymorphic human repeat DNA markers. Telomere function was evaluated by determining telomerase activity, telomere length, and telomere-repeat factors 1 and 2 (TRF1 and TRF2) expression.Results Patients with high microsatellite instability (MSI-H) gastric cancers showed a significantly better prognosis than those affected by microsatellite stable or low microsatellite instability (MSS/MSI-L) tumors (P=0.03). The lowest expression levels of TRF1 and TRF2 were associated with MSI-H gastric cancers (P=0.008 and 0.006, respectively). Moreover, a clear trend toward a worse prognosis was found in the group of patients who had tumors with the shortest telomeres (P=0.01). Cox multivariate analysis showed that MSI emerged as a protective prognostic factor; MSS/MSI-L tumors conferred a significantly poor prognosis in patients (relative risk=4.862-fold greater than the MSI-H group) (P=0.033). Telomere length of gastric tumors less than 2.86 kbp was a factor that led to a poor prognosis (relative risk=4.420, with respect to tumors showing telomere length≥2.86 kbp) (P=0.002).Conclusion We propose telomere status as a potential molecular marker with usefulness in the establishment of the prognosis of gastric cancers both for the mutator phenotype and for the suppressor pathway.
PB Lippincott, Williams & Wilkins
SN 0954-691X
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/34881
UL https://hdl.handle.net/20.500.14352/34881
LA eng
NO Fundación de Investigación Médica Mutua Madrileña
NO Santander-UCM
NO RTICC
DS Docta Complutense
RD 11 may 2025