RT Journal Article
T1 Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goats
A1 Juan Ferré, Lucía De
A1 Álvarez Sánchez, Julio
A1 Romero Martínez, Beatriz
A1 Bezos Garrido, Javier
A1 Castellanos, Elena
A1 Aranaz Martín, Alicia
A1 Mateos García, Ana Isabel
A1 Domínguez Rodríguez, Lucas José
AB Culture is considered the definitive technique for Johne's disease diagnosis, and it is essential for later applications of certain molecular typing techniques. In this study, we have tested four solid media (Herrold's egg yolk medium [HEYM] with sodium pyruvate and mycobactin [HEYMm-SP], HEYM with mycobactin and without sodium pyruvate [HEYMm], Middlebrook 7H11 with mycobactin [Mm], and Löwenstein-Jensen with mycobactin [LJm]) for isolation of Mycobacterium avium subsp. paratuberculosis strains in 319 tissue samples from cattle herds and goat flocks. We have shown that each of the two main groups of M. avium subsp. paratuberculosis (type II and type I/III) has different requirements for growth in the culture media studied. The recommended solid media for isolation of type I/III strains are LJm and Mm, since the combination of both media allowed the recovery of all these strains. The most widespread culture medium, HEYM, is not suitable for the isolation of this group of M. avium subsp. paratuberculosis strains. Regarding the type II strains, HEYMm-SP was the medium where more strains were isolated, but the other three media are also needed in order to recover all type II strains. The incubation period is also related to the strain type. In conclusion, because the type of strain cannot be known in advance of culture, coupled with the fact that cattle and goats can be infected with both groups of strains, we recommend the use of the four solid media and the prolongation of the incubation period to more than 6 months to detect paratuberculous herds/flocks and to determine the true prevalence of the infection.
PB American Society for Microbiology
SN 0099-2240
YR 2006
FD 2006-09
LK https://hdl.handle.net/20.500.14352/93519
UL https://hdl.handle.net/20.500.14352/93519
LA eng
NO de Juan, L., Alvarez, J., Romero, B., Bezos, J., Castellanos, E., Aranaz, A., Mateos, A., & Domínguez, L. (2006). Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goats. Applied and environmental microbiology, 72(9), 5927–5932. https://doi.org/10.1128/AEM.00451-06
NO Copyright © 2006, American Society for Microbiology. All Rights Reserved.Corresponding author. alaranaz@vet.ucm.esFinanciación: This work was funded by projects AGL2004-08092/GAN and AGL2005-07792/GAN of the Spanish Ministry of Science and Education and by the Spanish Ministry of Agriculture, Fisheries, and Food(MAPA).
NO Ministerio de Ciencia y Educación
NO Ministerio de Agricultura, Pesca y Alimentación
NO Project AGL2005-07792/GAN
NO Project AGL2004-08092/GAN
DS Docta Complutense
RD 1 sept 2024