RT Journal Article
T1 Sensitive electrochemical determination of miRNAs based on a sandwich assay onto magnetic microcarriers and hybridization chain reaction amplification
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Campuzano Ruiz, Susana
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Montoya Miñano, Juan José
A1 Pingarrón Carrazón, José Manuel
AB A novel electrochemical approach for determination of miRNAs involving a sandwich hybridization assay onto streptavidin-magnetic beads (Strep-MBs), hybridization chain reaction (HCR) amplification and amperometric detection at disposable screen-printed carbon electrodes is reported. Using miRNA-21 as the target analyte, a dynamic linear range from 0.2 to 5.0 nM with a 60 pM (1.5 fmol in 25 μL) detection limit was obtained. The achieved sensitivity is 24-fold higher than a non-HCR amplification approach involving conventional sandwich type assay onto MBs. Moreover, the whole assay time lasted 1 h 45 min which is remarkably shorter than other reported methodologies. The methodology exhibited full selectivity against other non-complementary miRNAs as well as an acceptable discrimination between homologous miRNA family members. The applicability of this novel approach was demonstrated by determining mature miRNA-21 in total RNA (RNAt) extracted from tumor cells and human tissues.
PB Elsevier
SN 0956-5663
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/96114
UL https://hdl.handle.net/20.500.14352/96114
LA eng
NO Torrente-Rodríguez, R.M., et al., Biosensors and Bioelectronics (2016), http://dx.doi.org/10.1016/j. bios.2016.07.003i
NO Torrente-Rodríguez, R. M., et al. «Sensitive Electrochemical Determination of miRNAs Based on a Sandwich Assay onto Magnetic Microcarriers and Hybridization Chain Reaction Amplification». Biosensors and Bioelectronics, vol. 86, diciembre de 2016, pp. 516-21. https://doi.org/10.1016/j.bios.2016.07.003.
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO CANAAN Research & Investment S.L. (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 17 abr 2025