RT Journal Article
T1 Sex-sorted bovine spermatozoa and DNA damage: I. Static features
A1 Gosálvez Berenguer, Jaime
A1 Ramírez, Miguel Ángel
A1 López Fernández, Carmen
A1 Crespo Castejón, Francisco
A1 Evans, Michael
A1 Kjelland, Michael
A1 Moreno, Juan Francisco
AB This study examined the static response of Spermatozoa DNA Fragmentation (SDF) after sex selection in bulls using a MoFlo® SX (Beckman Coulter, Miami FL) spermatozoa sorter to produce three different subpopulations: 1) Spermatozoa bearing X- chromosomes with a purity of 95%, 2) Spermatozoa bearing Y-chromosomes with a purity of 95%, and 3) non-viable spermatozoa. The static response of SDF refers to the baseline values observed for DNA damage when analyzed pre- and post sex-sorting. Results showed that while the baseline level SDF in pre-sorted bull spermatozoa samples ranged from 5.3% to 11% with an average of 7.9% ± 2.1%, the level of SDF obtained in X- and Y-chromosome sorted samples was much lower (3.1% ± 1.9%) and statistical differences were obtained after comparing both groups (P < 0.01). Spermatozoa containing a fragmented DNA molecule tend to be accumulated in the non-viable subpopulation. The baseline SDF level in X- and Y-chromosome sorted subpopulations is reduced, by 63% on average when compared to the values obtained in the neat semen sample. Different bulls exhibit unique SDF reduction efficiencies via the X- and Y-chromosome sex selection process.
PB Elsevier
SN 0093-691X
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/97424
UL https://hdl.handle.net/20.500.14352/97424
LA eng
NO Gosálvez J, Ramirez MA, López-Fernández C, Crespo F, Evans KM, Kjelland ME, Moreno JF. Sex-sorted bovine spermatozoa and DNA damage: I. Static features. Theriogenology. 2011 Jan 15;75(2):197-205
NO Ministerio de Ministerio de Educación y Ciencia (España)
DS Docta Complutense
RD 13 abr 2025