RT Journal Article
T1 Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging
A1 Erami, Roghayeh Sadeghi
A1 Ovejero, Karina
A1 Meghdadi, Soraia
A1 Filice, Marco
A1 Amirnasr, Mehdi
A1 Rodríguez Diéguez, Antonio
A1 Orden Hernández, María Ulagares De La
A1 Gómez Ruiz, Santiago
AB Functionalized magnetite nanoparticles (FMNPs) and functionalized mesoporous silica nanoparticles (FMSNs) were synthesized by the conjugation of magnetite and mesoporous silica with the small and fluorogenic benzothiazole ligand, that is, 2(2-hydroxyphenyl)benzothiazole (hpbtz). The synthesized fluorescent nanoparticles were characterized by FTIR, XRD, XRF, 13C CP MAS NMR, BET, and TEM. The photophysical behavior of FMNPs and FMSNs in ethanol was studied using fluorescence spectroscopy. The modification of magnetite and silica scaffolds with the highly fluorescent benzothiazole ligand enabled the nanoparticles to be used as selective and sensitive optical probes for zinc ion detection. Moreover, the presence of hpbtz in FMNPs and FMSNs induced efficient cell viability and zinc ion uptake, with desirable signaling in the normal human kidney epithelial (Hek293) cell line. The significant viability of FMNPs and FMSNs (80% and 92%, respectively) indicates a potential applicability of these nanoparticles as in vitro imaging agents. The calculated limit of detections (LODs) were found to be 2.53 × 10−6 and 2.55 × 10−6 M for Fe3O4-H@hpbtz and MSN-Et3N-IPTMS-hpbtz-f1, respectively. FMSNs showed more pronounced zinc signaling relative to FMNPs, as a result of the more efficient penetration into the cells.
PB MDPI
SN 2079-4991
YR 2018
FD 2018-06-14
LK https://hdl.handle.net/20.500.14352/12257
UL https://hdl.handle.net/20.500.14352/12257
LA eng
NO Received: 7 May 2018 / Revised: 24 May 2018 / Accepted: 12 June 2018 / Published: 14 June 2018(This article belongs to the Special Issue Nanostructured Biosensors)
NO Ministerio de Economía y Competitividad de España (MINECO)/FEDER
NO Centro de Excelencia Severo Ochoa
NO Universidad Rey Juan Carlos de Madrid/Banco de Santander
NO Isfahan University of Technology Research Council
NO Iran National Science Foundation
NO Ministerio de Ciencia e Innovación (MICINN)
NO Pro-CNIC Foundation (España)
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 21 abr 2025