RT Journal Article
T1 Inflammatory mediators alter interleukin-17 receptor, interleukin-12 and -23 expression in human osteoarthritic and rheumatoid arthritis synovial fibroblasts: Immunomodulation by vasoactive intestinal peptide
A1 Carrión Caballo, Mar
A1 Pérez García, Selene
A1 Jimeno, Rebeca
A1 Juarranz Moratilla, Yasmina
A1 González Álvaro, Isidoro
A1 Pablos Álvarez, José Luis
A1 Gutiérrez Cañas, Irene
A1 Pérez Gomáriz, Rosa María
AB Aims: To assess the contribution of fibroblast-like synoviocytes (FLS) to the inflammatory joint microenvironment under different pathogenic stimuli and their potential to respond to interleukin (IL)-17 and to determine whether the neuroimmunomodulatory vasoactive intestinal peptide (VIP) is able to modulate IL-17 receptor (IL-17R) and related cytokines. Methods: The effect of proinflammatory cytokines [tumor necrosis factor (TNF) and IL-17] and Toll-like receptor (TLR) ligands [poly(I:C) and lipopolysaccharide (LPS)] on IL-17R expression and IL-12 and IL-23 production was studied in osteoarthritis (OA)- and rheumatoid arthritis (RA)-FLS, involved in Th1Th17 differentiation. The effect of VIP was also determined. IL-17RA, IL-17RC, IL-12p35 and IL-23p19 expression was measured by real-time polymerase chain reaction. IL-12 and IL-23 protein levels were measured by ELISA in supernatant cultures. Results: TNF, LPS and poly(I:C) induced an increase in IL-17RA in RA-FLS, whereas TNF, TNF plus IL-17 and poly(I:C) enhanced IL-17RC transcripts in FLS. VIP diminished the upregulated expression of IL-17RA in RA-FLS following TNF and poly(I:C). TNF, LPS and poly(I:C) increased IL-12 and IL-23 levels in cells derived from patients presenting both pathologies. However, IL-17A decreased IL-12 and augmented IL-23. VIP decreased IL-12p35 mRNA upregulation by poly(I:C) and IL-23p19 transcripts in LPS-treated FLS. Conclusions: Inflammatory cytokines and TLR ligands modulate IL-17R, IL-12 and IL-23 possibly favoring the cross talk between FLS and Th1Th17 cells. The ability of VIP to counteract the enhancing effect of proinflammatory molecules on IL-17R and the IL-12 family of cytokines corroborates and amplifies the beneficial effect of this endogenous neuroimmunopeptide in rheumatic diseases.
PB Karger Publishers
SN 1021-7401
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/93435
UL https://hdl.handle.net/20.500.14352/93435
LA eng
NO Mar Carrión, Selene Pérez-García, Rebeca Jimeno, Yasmina Juarranz, Isidoro González-Álvaro, José Luis Pablos, Irene Gutiérrez-Cañas, Rosa P. Gomariz; Inflammatory Mediators Alter Interleukin-17 Receptor, Interleukin-12 and -23 Expression in Human Osteoarthritic and Rheumatoid Arthritis Synovial Fibroblasts: Immunomodulation by Vasoactive Intestinal Peptide. Neuroimmunomodulation 1 August 2013; 20 (5): 274–284. https://doi.org/10.1159/000350892
NO Instituto de Salud Carlos III
NO Comunidad de Madrid
NO European Commission
DS Docta Complutense
RD 5 abr 2025